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The use of tears for diagnosis of GM1 gangliosidosis. |
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| Authors: | A Tsuboyama F Miki M Yoshida Y Ogura T Miyatake |
| Affiliation: | 1. First Department of Internal Medicine, Jichi Medical School, Yakushiji, Tochigi, Japan,;2. Tokyo Metropolitan Institute of Medical Science, Honkomagome, Tokyo, Japan;3. Department of Neurology, Jichi Medical School, Yakushiji, Tochigi, Japan;4. Section of Preventive Hygiene, Tochigi Prefectural Office, Tochigi, Japan |
| Abstract: | The properties of beta-galactosidase of tears were investigated and the standard assay system was accomplished. The pH optimum was 4.2. The enzyme had a KM of 8.3 X 10(-4) M. The activity was stimulated by chloride ions and slightly stabilized by bovine serum albumin. The activities of normal individuals were 205 +/- 80 (S.D.) nmol/h/ml. The activity in the tears of the patient with GM1 gangliosidosis decreased to about 20% of normal control and this disease could be diagnosed by the assay of beta-galactosidase in tears. |
| Keywords: | Reprint requests should be addressed to: Dr.T. Miyatake Department of Neurology Jichi Medical School Yakushigi Tochigi Japan 329-04. |
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