1364例恶性肿瘤p53蛋白表达的检测及其临床病理学意义

目的：探讨p53基因异常表达在人癌发生过程的病理生物学作用和临床病理学意义。方法：对人体1364例不同类型恶性肿瘤（其中615例鳞癌，382例腺癌及367例其它恶性肿瘤）及相应良性病变组织进行p53蛋白表达的免疫组化（LASB法）检测。结果：恶性肿瘤组织p53蛋白表达的阳性检出率56．7％（773／1364）明显高于良性病变组织6．7％ （42／628）（P＜0．001）。结论：p53基因的异常表达（基因突变或／及蛋白积累）是人体多种恶性肿瘤病的普遍现象，可能是人癌发生发展过程中关键的变化之一。免疫组化检测p53蛋白过度表达可以作为区别良恶性病变有参考价值的指标之一。

Immunohistochemical Detection of p53 Protein Alterated Expression in 1 364 Patients with Maligant Tumors and Its Clinicopathological Significance

Objective: The aim of this study was to investigate the effect of p53 gene alterated expression on human carcinogenesis. Methods: p53 protein expression in 1 364 patients with malignant tumors and 628 benign lesions was detected by immunohistochemistry (LSAB). Results: The frenquency of p53 protein positive was significant higher in malignant tumors 56. 67% (733/1364) ] than those in related benign lesions 6. 7% (42/623) (P < 0. 001 ) ]; It was revealed the similar situation as compared squamous cell cancer (615 ), adenocarcinoma (382 ), and other types of malignancies (367 ) with their related benign lesions respectively (P < 0. 001 ). Conclusion: Alterated expression of p53 protein (gene multation or protein accumulation) may involve one of the critical events of human carcinogenesis. Detection of p53 overexpression may be a useful biomarkers for assessment of maligant tumors.