| Construction and Expression of Recombinant Plasmid pCD-rbFGF in Osteoblasts |
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| 作者姓名： | 杨探 杨述华 郭晓东 屈伸 |
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| 作者单位： | YANG Cao，YANG Shuhua，GUO Xiaodong Department of Orthopedics，Xiehe Hospital，Tongji Medical College，Huazhong University of Science and Technology，Wuhan 430022 QU Shen Department of Biochemistry，Tongji Medical College，Huazhong University of |
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| 摘 要： | Basicfibroblastgrowth factor(b FGF) exertsbi-ological effects on a wide range of mesoderm- andneuroectoderm- derived cells,acting as morphogensand mitogens.In addition,b FGF plays a crucial rolein wound- healing process.It stimulatesthe prolifera-tion ofalltypes ofcellsinvolved in the wound- healingprocess both in vitro and in vivo.In tissues such ascartilage and bone,b FGF has been reported to pro-mote chondrossification and play a role in the growthof osseous tissue1] .To explore the po…
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| 收稿时间： | 20 October 2001 |
Construction and expression of recombinant plasmid pCD-rbFGF in osteoblasts |
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| Yang Cao,Yang Shuhua,Guo Xiaodong,Qu Shen.Construction and Expression of Recombinant Plasmid pCD-rbFGF in Osteoblasts[J].Journal of Zuazhong University of Science and Technology: Medical Edition,2002,22(2):109-111. |
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| Authors: | Yang Cao Yang Shuhua Guo Xiaodong Qu Shen |
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| Institution: | 1. Department of Orthopedics, Xiehe Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022
2. Department of Biochemistry, Tong ji Medical College, Huazhong University of Science and Technology, Wuhan 430030 |
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| Abstract: | Summary: To construct basic fibroblast growth factor (bFGF) eukaryotic expression vector and to e-valuate the possibility of bFGF gene therapy in orthopedic disease, the pCD-rbFGF recombinant plas mid was constructed by cloning rat basic fibroblast growth factor (bFGF) cDNA into an eukaryotic expression vector, pcDNA3. Rat osteoblasts were transfected with pCD-rbFGF plasmid by lopofectin mediated gene transfer, the transient expression was detected by streptavidin-biotin-enzyme complex (SABC) method. It was observed that the expression of rat bFGF gene was detected 72 h after transfected distinctly. Basic fibroblast growth factor gene therapy is a method of potential for a wide array of orthopedic diseases. |
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| Keywords: | basic fibroblast growth factor recombinant plasmid gene transfer gene expression |
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