| Adenovirus-mediated Transfer of p53 and p16Inhibiting Proliferating Activity of Human Bladder Cancer Cell EJin vitro and in vivo |
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| 引用本文： | 朱朝辉,邢诗安,林晨,曾甫清,鲁功成,付明,张雪艳,梁萧,吴旻. Adenovirus-mediated Transfer of p53 and p16Inhibiting Proliferating Activity of Human Bladder Cancer Cell EJin vitro and in vivo[J]. 华中科技大学学报(医学英德文版), 2002, 0(4) |
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| 作者姓名： | 朱朝辉 邢诗安 林晨 曾甫清 鲁功成 付明 张雪艳 梁萧 吴旻 |
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| 作者单位： | DepartmentofUrology，CentralLaboratory XieheHospital，NationalLaboratoryofMolecularOncology，DepartmentofUrology，DepartmentofUrology，NationalLaboratoryofMolecularOncology，NationalLaboratoryofMolecularOncology，NationalLaboratoryofMolecularOncology，NationalLaboratoryofMolecularOncology TongjiMedicalCollege，HuazhongUniversityofScienceandTechnology，Wuhan430022，CancerInstituteofChineseAcademyofMedicalSciences，Beijing100021，CancerInstituteofChineseAcademyofMedicalSciences，Beijing100021，CancerInstituteofChineseAcademyofMedicalSciences，Beijing100021，CancerInstituteofChineseAcademyofMedicalSciences，Beijing100021，CancerInstituteofChineseAcademyofMedicalSciences，Beijing100021 |
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| 基金项目： | Thisprojectwassupported by a grant from 86 3Project ofChina(No.Z2 0 - 0 1- 0 2 ) |
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| 摘 要： | Previous studies have shown the tumor sup-pressor genes p5 3and p1 6were mutated or defect-ed in70 % and73% of the transurethral resectionbladder tumor( TURBt) specimens respectively[1] .The aim of this study was to evaluate the effects ofadenovirus ( Ad) - mediated transfer of p5 3and p1 6on the proliferating activity of human bladder can-cer cells EJboth in vitro and in vivo.1 MATERIAL SAND METHODS1 .1 Cell Lines and Adenoviral VectorsThe human bladder cancer cell line EJwas …
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Adenovirus-mediated Transfer of p53 and p16Inhibiting Proliferating Activity of Human Bladder Cancer Cell EJin vitro and in vivo |
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| ZHU Zhaohui ,XING Shian ,L IN Chen. Adenovirus-mediated Transfer of p53 and p16Inhibiting Proliferating Activity of Human Bladder Cancer Cell EJin vitro and in vivo[J]. Journal of Huazhong University of Science and Technology. Medical sciences, 2002, 0(4) |
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| Authors: | ZHU Zhaohui XING Shian L IN Chen |
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| Affiliation: | ZHU Zhaohui 1,XING Shian 2,L IN Chen |
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| Abstract: | To evaluate the effects of adenovirus (Ad) - mediated transfer of p5 3and p16 on hum an bladder cancer cells EJ,EJwere transfected with Ad- p5 3and Ad- p16 . Cell growth,m orphologi- cal change,cell cycle,apoptosis were measured using MTT assay,flow cytom etry,cloning form a- tion,im munocytochemical assays.Ad- p16 or Ad- p5 3alone could inhibit the proliferating activity of EJcells in vitro.Ad- p5 3could induce apoptosis of partial EJcells.G1arrest was observed72 h after infection with Ad- p16 ,but apoptosis was not obvious.The transfer of Ad- p16 and Ad- p5 3 could significantly inhibit the growth of EJcells,decrease the cloning formation rate and induce apoptosis of large num ber of EJcells. The occurrence time of subcutaneous tumor was delayed and the tum or volume in 4 weeks was dim inished by using Ad- p5 3com bined with Ad- p16 and the dif- ference was significant com pared with using Ad- p5 3or Ad- p16 alone.It was suggested that the transfer of wild- type p5 3and p16 could significantly inhibit the growth of human bladder cancer in vitro and in vivo. |
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| Keywords: | p5 3 p16 bladder cancer adenovirus |
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