| Experimental Study of Plasmid TGF-β1 DNA Gene Transfer with Lipofectamine into Rabbit Corneal Epithelial Cells In Vitro |
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| 引用本文： | 黄琼,胡燕华,姜发纲,陈宏.Experimental Study of Plasmid TGF-β1 DNA Gene Transfer with Lipofectamine into Rabbit Corneal Epithelial Cells In Vitro[J].华中科技大学学报(医学英德文版),2002,22(1). |
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| 作者姓名： | 黄琼 胡燕华 姜发纲 陈宏 |
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| 作者单位： | HUANG Qiong，HU Yanhua，JIANG Fagang，CHEN Hong Department of Ophthalmology，Xiehe Hospital，Tongji Medical College，Huazhong University of Science and Technology，Wuhan 430022 |
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| 基金项目： | This project was supported by a grant from the Natural
Sciences Foundation of Hubei Province (N0 .97J0 70 ) |
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| 摘 要： | To investigate whether the TGF-β1 plasmid DNA carried by lipofectamine could be introduced into cultured rabbit corneal epithelial cells, specific expression of the plasmid pMAM TGF-β1in the cultured corneal epithelial cells was studied. Two days after 12 h of transfection of pMAMTGF-β1 mediated by lipofectamine into the cultured corneal epithelial cells, the TGF-β1 protein expression specific for pMAMTGF-β1 in the cells was detected by means of immunohistochemical staining and the positive rate was 23. 37 %. The results suggested that foreign plasmid DNA could be effectively delivered into cultured rabbit corneal epithelial cells by means of lipofectamine, and this will provide a promising method of studying TGF-β1 on the mechanism of physiology and pathology concerned with corneal epithelial cells.
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Experimental Study of Plasmid TGF-β1 DNA Gene Transfer with Lipofectamine into Rabbit Corneal Epithelial Cells In Vitro |
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| HUANG Qiong,HU Yanhua,JIANG Fagang,CHEN Hong.Experimental Study of Plasmid TGF-β1 DNA Gene Transfer with Lipofectamine into Rabbit Corneal Epithelial Cells In Vitro[J].Journal of Zuazhong University of Science and Technology: Medical Edition,2002,22(1). |
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| Authors: | HUANG Qiong HU Yanhua JIANG Fagang CHEN Hong |
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| Institution: | Department of Ophthalmology, Xiehe Hospital, Tongji Medical College, Huazhong University of Science anti Technolg y, Wuhan 430022 |
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| Abstract: | To investigate whether the TGF-β1 plasmid DNA carried by lipofectamine could be introduced into cultured rabbit corneal epithelial cells, specific expression of the plasmid pMAM TGF-β1in the cultured corneal epithelial cells was studied. Two days after 12 h of transfection of pMAMTGF-β1 mediated by lipofectamine into the cultured corneal epithelial cells, the TGF-β1 protein expression specific for pMAMTGF-β1 in the cells was detected by means of immunohistochemical staining and the positive rate was 23. 37 %. The results suggested that foreign plasmid DNA could be effectively delivered into cultured rabbit corneal epithelial cells by means of lipofectamine, and this will provide a promising method of studying TGF-β1 on the mechanism of physiology and pathology concerned with corneal epithelial cells. |
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| Keywords: | cornea epithelial cells gene transfer transforming growth factor beta |
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