| UVA与UVB对人体黑素细胞生长的影响 |
| |
| 引用本文： | 潘建英,竺逸,张玉彬,沈光祖,金泰廙,金锡鹏.UVA与UVB对人体黑素细胞生长的影响[J].劳动医学,2009(1):43-45. |
| |
| 作者姓名： | 潘建英 竺逸 张玉彬 沈光祖 金泰廙 金锡鹏 |
| |
| 作者单位： | 复旦大学公共卫生学院; |
| |
| 摘 要： | 目的]探讨长波紫外线（UVA）和中波紫外线（UVB）照射人体黑素细胞作用的差异。方法]以不同剂量UVA和UVB分别照射黑素细胞，用四甲基偶氮唑盐微量酶反应比色法（MTT）测量细胞增殖率，比色法测定酪氨酸酶活性，吸光度测定黑素含量的变化。结果]人体黑素细胞经0-2．25J／cm^2UVA照射后，黑素含量和酪氨酸酶活性无明显改变，其中较高剂量UVA（〉1．8J／cm^2）可使黑素细胞存活率下降。0-0．06J／cm^2UVB能明显抑制黑素细胞存活率，并能激活酪氨酸酶和促进黑素细胞的黑素合成。结论]离体水平UVB对黑素细胞的促黑素合成作用明显强于UVA。
|
| 关 键 词： | 长波紫外线 中波紫外线 黑素细胞 |
Influence of UVA and UVB on Human Melanocytes Growth |
| |
| PAN Jian-ying,ZHU Yi,ZHANG Yu- bin,SHENG Guang-zu,JIN Tai-yi,JIN Xi-peng.Influence of UVA and UVB on Human Melanocytes Growth[J].Journal of Labour Medicine,2009(1):43-45. |
| |
| Authors: | PAN Jian-ying ZHU Yi ZHANG Yu- bin SHENG Guang-zu JIN Tai-yi JIN Xi-peng |
| |
| Institution: | School of Public Health;Fudan University;Shanghai 200032;China |
| |
| Abstract: | Objective]To investigate the distinct response between human melanocytes separately exposed to UVA and UVB.Methods]The proliferation rate of melanocytes was determined by MTT assay.The viability of intracellular tyrosinase was determined by photocolorimetry.The levels of intracellular melanin was evaluated by spectrophotometry.Results]Human normal melanocytes irradiated with 0-2.25J/cm2 UVA showed no marked changes while high dose UVA(>1.8J/cm2)caused an obvious drop in cell proliferation.0-0.06 J/cm2 UV... |
| |
| Keywords: | UVA UVB melanocyte |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
