肝细胞癌中MyD88和STAT3的表达及其意义

目的 探讨髓样分化因子88(MyD88)和转录激活因子3(STAT3)在肝细胞癌(HCC)组织中的表达及其生物学意义.方法 采用免疫组化方法 检测MyD88和STAT3在82例肝痛组织及其对应的癌旁肝组织中的表达水平.结合肝癌临床病理指标分析它们的相关性.结果 MyD88和STAT3蛋白在癌组织中的阳性表达率分别为67.1%(55/82)和69.5%(57/82),高于癌旁肝组织中的11.0%(9/82)和8.5%(7/82).差异均具有统计学意义(P＜0.05).在肝癌组织中MyD88与STAT3的阳性表达呈正相关(r=0.578,P=0.002).MyD88和STAT3表达与HCC患者的性别、癌的分化程度、有无HBV感染和肝硬化有关;上述4指标分组间差异具有显著性(P＜0.05).而与HCC肿瘤大小及有无静脉浸润无关;该2指标分组间差异无显著性(P＞0.05).结论 MyD88和STAT3表达上调,导致肝癌细胞增殖和免疫逃逸是肝癌发生发展的重要分子机制.

The expression and significance of MyD88 and STAT3 in hepaticullar cancinoma

Objective：To study the expression of MyD88 and STAT3 in hepatocellular cancer (HCC) tissues and its signicficance.
Methods：S-P immunohistological assay was applied to detect the expression of MyD88 and STAT3 protein in specimens from hepatocellular carcinoma tissues and adjacent liver tissues of 82 HCC cases. Correlation analysis was used to determine the relation of MyD88 and STAT3 with the clinicopathological data.
Results：The positive rate of MyD88 and STAT3 expression was 67.1% and 69.5% respectively in 82 hepatocellular carcinoma tissues, which were significantly higher than the positive rate (11% and 8.5% respectively) in adjacent tissues（P＜0.05). STAT3 expression was positively correlated with MyD88 (r=0.578，P=0.002).  MyD88 and STAT3 expression were correlated with patient′s gender, differitiation of HCC, HBV infection and cirrhosis (P＜0.05), but not with lumor size, venous invasion (P>0.05).
Conclusions：MyD88 and STAT3 genes are significantly over expressed in HCC tissues and may promote the initiation and development of HCC by cell proliferation and immune escape.