饮食中亚硝酸盐对大鼠肝缺血再灌注损伤的保护作用

目的 探讨饮食中亚硝酸盐对大鼠肝脏缺血再灌沣(I/R)损伤的保护作用.方法 Wistar 大鼠随机分为I/R组(对照组)、亚硝酸钠预处理组(实验组)和假手术组.建立大鼠肝脏部分I/R模型,实验组的饮水中加入少量亚硝酸盐并喂养7 d后建模,检测血清谷丙转氨酶(ALT)和谷草转氨酶(AST)的水平,测定肝组织中超氧化物歧化酶(SOD),丙二醛(MDA),一氧化氮(NO),一氧化氮合酶(NOS)含量及活性.用Hoechst染色法检测细胞凋亡,并观察各组病理形态学的改变.结果 对照组AST,ALT,MDA水平和细胞凋亡的程度均明显异常.实验组上述指标的异常变化均明显减轻,NO明显高于对照组,差异有统计学意义(P＜0.01);但NOS并未增多,与对照组差异无统计学意义.结论 饮食中的亚硝酸盐对大鼠肝脏I/R损伤有明显的保护作用.

The protective effect of dietary nitrite supplements on hepatic ischemia-reperfusion injury in rats

Objective：To investigate the effectiveness of dietary nitrite supplements  on hepatic ischemia-reperfusion(I/R) injury in rats. 
Methods：Wistar rats were randomly divided into 3 groups: I/R group, nitrite precondition group(N group) and sham operation group (S group). Ischemia and reperfusion of liver was conducted in the I/R group and N group.In N group, nitrite was added to the drinnking water and given to rats at 7 days before making I/R model. In the 3 groups, the level of serum alanine aminotransferase (ALT), aspartate aminotransferase (AST), and the content of superoxide dismutase (SOD), hepatic tissue malondialdehyde(MDA) and nitric oxide (NO),nitric oxide synthase (NOS) were determined; cell apoptosis was detected by Hoechst staining and liver histopathological changes were observed.Results：The levels of serum AST, ALT, MDA and apoptosis in I/R group were markedly abnormal; but in N group, the above mentioned abnormal changes were significantly reduced and the level of NO was significantly higher as compared with I/R group (P<0.01), while NOS remained relatively unchanged in N group.
Conclusions：Dietary nitrite has significant protective effect on the hepatic ischemia-reperfusion injury in rats.