| 间充质干细胞定向成骨细胞分化的蛋白质组学分析 |
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| 引用本文： | 刘元林,于晓妉,刘晓丹,李秀森,毛宁.间充质干细胞定向成骨细胞分化的蛋白质组学分析[J].第四军医大学学报,2006,27(17):1551-1554. |
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| 作者姓名： | 刘元林 于晓妉 刘晓丹 李秀森 毛宁 |
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| 作者单位： | 军事医学科学院基础医学研究所,北京,100850;军事医学科学院基础医学研究所,北京,100850;军事医学科学院基础医学研究所,北京,100850;军事医学科学院基础医学研究所,北京,100850;军事医学科学院基础医学研究所,北京,100850 |
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| 基金项目： | 国家重点基础研究发展计划(973计划) |
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| 摘 要： | 目的:探讨用蛋白质组学方法分析人骨髓间充质干细胞(MSCs)定向成骨细胞诱导分化中细胞蛋白表达谱的改变. 方法: 从人骨髓细胞中分离获得MSCs,经细胞表型及多向分化能力测定鉴定MSCs的纯度和功能后,采用定向成骨细胞分化诱导培养体系,于分化后14 d分别提取未分化细胞及分化后细胞的总蛋白,双向电泳分析,确定蛋白差异点,经酶切后行蛋白肽质量指纹谱鉴定差异蛋白表达. 结果: 双向电泳找到38个蛋白差异点,质谱分析鉴定出23个差异蛋白分子表达. 结论: 用蛋白质组学方法可高通量筛选与MSCs定向诱导成骨细胞分化相关的重要蛋白.
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| 关 键 词： | 蛋白质组学 间质干细胞 蛋白表达 |
| 文章编号： | 1000-2790(2006)17-1551-04 |
| 收稿时间： | 04 3 2006 12:00AM |
| 修稿时间： | 05 18 2006 12:00AM |
Proteomic analysis of human bone marrow-derived mesenchymal stem cells induced to differentiate into osteoblasts |
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| LIU Yuan-Lin,YU Xiao-Dan,LIU Xiao-Dan,LI Xiu-Sen,MAO Ning.Proteomic analysis of human bone marrow-derived mesenchymal stem cells induced to differentiate into osteoblasts[J].Journal of the Fourth Military Medical University,2006,27(17):1551-1554. |
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| Authors: | LIU Yuan-Lin YU Xiao-Dan LIU Xiao-Dan LI Xiu-Sen MAO Ning |
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| Institution: | Institute of Basic Medical Sciences, Academy of Military Medical Sciences, Beijing 100850, China |
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| Abstract: | AIM: To analyze the difference of protein profiles between human bone marrow-derived mesenchymal stem cells (MSCs) and osteogenic differentiated MSCs by proteomic approach. METHODS: The MSCs were isolated primarily from bone marrow of adults and detected in cell purity by flow cyto- metry analyzing cell phenotypes and in cell function by multi- lineage potential test. The undifferentiated MSCs and osteogenic differentiated MSCs over a period of 14 d were collected and cell total proteins were extracted for finding out the differential expression by proteomic analysis including 2-D gel electrophoresis and Peptide Mass Fingerprinting technology. RESULTS: Twenty-three differentially expressed proteins were identified by MALDI-TOF-MS analysis. CONCLUSION: Proteomic approach is a high throughput method to screen the key proteins which play important roles in MSCs osteogenic differentiation. |
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| Keywords: | proteomics mesenchymal stem cells protein expression |
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