| 白首乌的组织培养和快速繁殖技术研究 |
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| 引用本文： | 嵇恂,高山林,黄和平.白首乌的组织培养和快速繁殖技术研究[J].药物生物技术,2010(2). |
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| 作者姓名： | 嵇恂 高山林 黄和平 |
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| 作者单位： | 中国药科大学中药学院; |
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| 摘 要： | 建立和优化白首乌组织培养克隆化快速繁殖技术,为保护白首乌的野生资源,发展人工资源和探讨育种新途径奠定基础。在组织培养条件下进行白首乌愈伤组织的诱导、分化,试管苗复壮和生根等一系列技术优化试验。在培养基中适当添加矮壮素(PP333)能显著改善试管苗的素质,萘乙酸(NAA)对植株愈伤组织的生成具有十分有效的作用。PP333和NAA与激素配合使用,能十分有效地调控白首乌愈伤组织的分化、试管苗的生长与生根。
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| 关 键 词： | 白首乌 组织培养 快速繁殖 生根培养 |
Tissue Culture and Rapid Propagation of Bunge Swallowwort |
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| JI Xun,GAO Shan-lin,HUANG He-ping.Tissue Culture and Rapid Propagation of Bunge Swallowwort[J].Pharmaceutical Biotechnology,2010(2). |
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| Authors: | JI Xun GAO Shan-lin HUANG He-ping |
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| Institution: | JI Xun,GAO Shan-lin,HUANG He-ping(School of Chinese Traditional Medicine,China Pharmaeutical University,Nanjing 210038,China) |
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| Abstract: | To establish and to optimize the technipue of clonig and rapid propagation by tissue culture in Bunge Swallowwort for the protection and development of naturd resource as well as seeking for new breeding way of B.Swallowwort by biotechnology,the research on the optimized technology of tissue culture including callus induction,differentiation,plantlet propagation,and rooting was designed.PP333 has significant stocky effect on the plantlets and NAA has the significant effect on the conformation of callus.PP33... |
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| Keywords: | Bunge swallowwort(Cynanchum auriculatum Royle ex Wight) Tissue culture Rapid propagation Rooting culture |
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