胚胎脊髓不同移植方法对成年大鼠损伤脊髓神经元的影响

目的：探讨胚胎脊髓不同移植方法防止成年鼠脊髓轴突损伤后引起的神经元萎缩的作用。方法：采用Wistar成年大鼠腰脊髓半切洞损伤模型。将实验动物分为五组：A组为正常对照组，B组为损伤组；C组为损伤＋移植组；D组为损伤＋移植＋椎旁肌组；E组为损伤＋移植＋大网膜组，手术后应用行为学和电生理检查观察大鼠神经功能恢复情况，应用尼氏染色方法观察神经元的大小,采用计算机图像分析技术，进行定量分析。结果 胚胎脊髓不同移植方法均可以防止老年鼠脊髓轴突损伤引起的神经元萎缩，图像分析发现其作用为E组＞D组＞C组＞B组＞A组，尤以E组效果最好，可以完全恢复损伤神经元的形态，大鼠神经功能的恢复也出现了相同的变化趋势。结论 鼠胚胎脊髓不同移植方法能维持神经元的细胞形态，对成年大鼠损伤脊髓功能恢复有促进作用。

EFFECTS OF DIFFERENT METHODS OF FETAL SPINAL CORD TISSUE TRANSPLANTED ON REVERSING THE AXOTOMY-INDUCED NEURONS ATROPHY RATS INJURED SPINAL CORD

Objective To explore the effects of different methods of fetal spinal cord(FSC) tissue transplanted on reversing the axotomy-induced neurons atrophy of adult rats injured spinal cord. Methods One hundred and twenty adult rats received lumbar spinal cord hemisection. Experimental rats were divided into five groups, the control group(Group A); spinal cord hemisection only(Group B); spinal cord hemisection plus FSC transplant (Group C); spinal cord hemisection plus FSC transplant plus pedicled paraspinal muscle(Group D); spinal cord hemisection plus FSC transplant plus pedicled omentum (Group E). Combined behavioral score(CBS), somatosensory evoked potentials (SEP), motor evoked potentials(MEP) were examined to evaluate the recovery of neurological function after operation. Rats were sacrificed after 1, 4 and 12 weeks. Nissl stained section was used for neurons quantitative image analysis. The positive cells were quantitative analysis by computer image analysis system. Results The different methods of FSC tissue transplantation could prevent the neurons atrophy secondary to axon injury of spinal cord in adult rats. The size of neurons were observed in five groups, they were group E > group D >group C > group B > group A( P< 0.05). Those increases in size of neurons were paralleled with a significant improvement in neurological function recovery. Conclusion It indicates that the different methods of FSC tissue transplantation can maintain the neurons morphology and improve the neurological function of rats.