Hepatitis B virus infection and replication in primary cultured human granulosa cells |
| |
Authors: | Jin Yan Ye Feng Shi Juanzi Qiu Hongtao Zhao Yingren Lin Shumei Chen Tianyan Liu Min He Yingli Zhang Shulin |
| |
Institution: | (1) Department of Infectious Disease, The First Hospital of Xi’an Jiaotong University, Jiankang Road 1#, Xi’an, 710061 Shaanxi, China;(2) Reproductive Center, Shaanxi Maternal and Child Care Service Hospital, Xi’an, China; |
| |
Abstract: | To investigate the infection and replication of hepatitis B virus (HBV) in primary cultured human granulosa cells. Human granulosa
cells were cultured with HBV-positive serum. Media were collected and assayed for HBsAg and HBeAg by ELISA, and HBV DNA by
quantitative PCR. HBsAg and HBcAg were detected by immunocytochemistry in cultured cells. HBV DNA and RNA were extracted and
amplified by nested PCR. Intracellular HBV DNA was localized by in situ hybridization. By co-cultivation of human GCs with
HBV-positive serum, a system was established to study HBV infection and replication in GCs. HBsAg in medium could be detected
from 4 to 96 h, and HBV DNA could be detected from 12 to 96 h after exposure. HBsAg and HBcAg showed positive signals by immunocytochemistry.
A 206-bp fragment was amplified by nested PCR to detect HBV DNA and RNA in granulosa cells. HBV DNA was detected in GC nuclei
by in situ hybridization. HBV can infect and replicate in human primary granulosa cells. This culture system could enable
us to study infection of ova by HBV. |
| |
Keywords: | |
本文献已被 PubMed SpringerLink 等数据库收录! |
|