转染野生型p53基因对肝癌细胞生长的影响

目的：通过转染野生型p53基因致肝癌细胞株BEL-7404，研究其对肝癌细胞的生长及凋亡作用。方法：野生型p53基因通过脂质体Lipofectamine介导转染人肝癌细胞株BEL-7404。用四甲基偶氮唑（MTT）法测定细胞生长曲线，用流式细胞仪进行细胞凋亡分析。结果：肝癌细胞BEK-7404转染野生型p53基因后，其生长作用明显受到抑制．细胞生长抑制率在第4天可达50．8％。流式细胞仪分析细胞凋亡结果：阴性对照组为1．7％，转染pUHD10-3组为3．2％．转染pUHD10-3-p53组为40．7％。结论：野生型p53基因可诱导肝癌细胞BEL-7404的生长抑制及发生凋亡。

EFFECT OF p53 GENE TRANSFECTION ON HEPATOCARCINOMA CELL

Objective:To investigate the effect of p53 gene transfection on the growth and apoptosis of hepatocarcinoma cell line BEL-7404.Methods:The HCC line BEL-7404 was transfected with plasmid pUHD10-3 recombined with wt-p53 gene mediated by Lipofectamine. 3-(4,5-Dimethy-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium-bromid (MTT) technique was used to analyse the growth of HCC cells. The apoptosis of the cells was evaluated by flow cytometry.Result:The growth of HCC cell line BEL-7404 was inhibited after transfected with wt-p53 gene, and 50.8% of the cells were inhibited on the fourth day. The rates of apoptosis measured by flow cytometry were: 1.7% in the untreated group, 3.2% in the group transfected with pUHD10-3,40.7% in the group tranfected with pUHD10-3-p53.Conclusion:The inhibition efficiency and apoptosis of hepatocarcinoma cell line BEL-7404 was induced by wt-p53 gene.