灯盏花素对大鼠2型糖尿病胰岛素抵抗的影响

目的:探讨灯盏花素(breviscapine,Bre)对2型糖尿病胰岛素抵抗大鼠模型的血糖、血脂和对胰岛素抵抗的影响。方法:高脂高糖结合链脲佐菌素(STZ)制备2型糖尿病胰岛素抵抗模型。4周后选取空腹血糖﹥16.7 mmol·L-1的大鼠随机分为模型组、Bre低、高剂量组(100,200 mg·kg-1·d-1),并设正常对照组。连续给药14 d后,断尾采血测血糖(FBG),空腹胰岛素(FINS),血清总胆固醇(TC),甘油三酯(TG),游离脂肪酸(FFA),计算胰岛素抵抗指数(HOMA-IR)。HE染色观察胰腺和心脏组织形态学改变。结果:与正常对照组比较,模型组FBG,FINS,TG,TC,FFA,HOMA-IR明显升高(P<0.05),显示胰岛素抵抗特征。与模型对照组相比,Bre高、低剂量组FBG,FINS,TG,TC,FFA,HOMA-IR降低(P<0.05);组织HE染色光镜下观察显示,模型组大鼠胰腺及心脏结构异常,Bre高、低剂量组均能明显改善胰腺及心脏组织的病理改变。结论:灯盏花素对2型糖尿病胰岛素抵抗大鼠模型有降低血糖水平、调节血脂紊乱的作用并呈剂量依赖性,可以改善胰岛素的抵抗,减轻胰腺和心脏的病理损伤。

Effect of Breviscapine in Type 2 Diabetes Mellitus Rats with Insulin Resistance

Objective: To study the effects of breviscapine(Bre) on serum glucose, serum lipid and insulin resistance in type 2 diabetes mellitus rats with insulin resistance and find its mechanism. Method: Experimental diabetic insulin resistance was duplicated in rats by feeling on high-fat and high-carbohydrate diet four weeks plus injecting low-does streptozotocin(STZ, 40 g· L^-1).Then the rats whose free blood glucose (FBG) level was equals to or surpasses 16.7 mmol· L^-1 were selected and divided into model group of Bre, the low does group, and the high does group. The medication was given for 14 days. FBG, fasting insulin(FINS), free fatty acids(FFA), triglycerides(TG), total cholesterol (TC) were measured and calculated the insulin resistance index(HOMA-IR) after 14 days.The pancreas, myocardial tissues were stained by HE so as to observe its morphologic changes. Result: FBG, FINS, TG, TC, FAA, HOMA-IR increased in the model group as compared with those in nomal group. FBG, FINS, TG, TC, FAA, HOMA-IR reduced in the Bre group compared with those in model group. Curative effect of Bre-H group was better than Bre-L group (P<0.05).Through HE-stain in model group the organizational structure of the pancreas and myocardial tissues was abnormal. The pathologic changes in different does Bre groups were significantly improved. Conclusion: Bre can reduce levels of blood glucose, regulate components and content of blood lipids, and improve the insulin resistance.