血管内皮生长因子及其受体在DR患者玻璃体中的表达

目的：观察血管内皮生长因子(vascular endothelial cell growth factor， VEGF)及其受体在糖尿病视网膜病变(diabetic retinopathy， DR)患者玻璃体中的表达，探讨其在DR发生发展中的作用。

方法：对13例16眼DR患者和15例15眼健康人，采用免疫组织化学法检测其玻璃体新生血管膜的VEGF及其受体(fms-样酪氨酸激酶， Flt-1和含激酶插入区受体， KDR)表达情况，同时采用酶联免疫吸附法(ELISA)检测DR患者玻璃体中VEGF、Flt-1和KDR的浓度。

结果：免疫组织化学染色显示，DR患者玻璃体血管膜中VEGF、Flt-1和KDR高度表达，玻璃体中VEGF、Flt-1和KDR浓度明显高于对照组(P<0.05或P<0.01)。

结论：VEGF及其受体Flt-1、KDR在DR患者玻璃体中高度表达，与DR患者的微血管生成关系密切，提示VEGF及其受体在DR的发生发展中起重要作用。

Expression of vascular endothelial cell growth factor and its receptors in vitreous of patients with diabetic retinopathy

AIM:To investigate the expression of vascular endothelial cell growth factor(VEGF)and its receptors in vitreous of patients with diabetic retinopathy(DR), and to discuss its role in the development of DR.

METHODS: Selected 13 patients(16 eyes)with DR and 15 healthy people(15 eyes), the expression of VEGF and its receptors(fms-like tyrosine kinase-1, Flt-1 and kinase insert domain containing receptor, KDR)were evaluated by immunohistochemistry in vitreous. The levels of VEGF, the Flt-1 and KDR in vitreous of patients with DR were examined with enzyme linked immunosorbent assay(ELISA).

RESULTS: Immunohistochemical staining showed that the expression of VEGF, Flt-1 and KDR in vitreous vessel membrane of patients with DR was increased significantly. And the levels of VEGF, Flt-1 and KDR in vitreous of patients with DR were obviously higher than that in the control group(P<0.05 or P<0.01).

CONCLUSION: VEGF, Flt-1 and KDR were widely expressed in vitreous of patients with DR, and were positively related to micro-angiogenesis of DR patients. It proved that VEGF and its receptors played important roles in the occurrence and development of DR.