| 利用小动物SPECT/CT和99m锝-葡糖二酸评估急性缺血坏死心肌的动物实验研究 |
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| 引用本文： | 宝璐尔,张林,周晓慧,孟庆姝,占贞贞,范慧敏.利用小动物SPECT/CT和99m锝-葡糖二酸评估急性缺血坏死心肌的动物实验研究[J].中国比较医学杂志,2014,24(7):14-18. |
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| 作者姓名： | 宝璐尔 张林 周晓慧 孟庆姝 占贞贞 范慧敏 |
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| 作者单位： | 同济大学附属东方医院心脏外科, 上海 200120;同济大学附属东方医院心脏外科, 上海 200120;同济大学附属东方医院心衰专科, 上海 200120;同济大学附属东方医院心衰研究所, 上海 200120;同济大学附属东方医院心脏外科, 上海 200120;同济大学附属东方医院心衰专科, 上海 200120;同济大学附属东方医院心衰研究所, 上海 200120;同济大学附属东方医院心脏外科, 上海 200120;同济大学附属东方医院心衰专科, 上海 200120;同济大学附属东方医院心衰研究所, 上海 200120;同济大学附属东方医院心脏外科, 上海 200120;同济大学附属东方医院心衰专科, 上海 200120;同济大学附属东方医院心衰研究所, 上海 200120;同济大学附属东方医院心脏外科, 上海 200120;同济大学附属东方医院心衰专科, 上海 200120;同济大学附属东方医院心衰研究所, 上海 200120 |
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| 基金项目： | 国家自然科学基金（81070073，81273263）；上海市医学重点专科（ZK2012A27）；上海领军人才（2012-053）；上海市浦东新区卫生系统领先人才培养计划资助（PKR2011-01）。 |
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| 摘 要： | 目的 利用99m锝-葡糖二酸（99mTc-Glucarate）和小动物SPECT/CT评估大鼠急性心肌缺血再灌注损伤后缺血坏死心肌的位置和范围。方法 建立大鼠急性缺血再灌注损伤模型，手术1 d后尾静脉注射99mTc-Glucarate，注射30 min后利用小动物SPECT/CT融合技术分析99m锝-葡糖二酸标记的心肌组织的位置和范围，并与氯化三苯基四氮唑（triphenyltetrazolium chloride，TTC）染色法标记的缺血坏死心肌比较。结果 小动物SPECT/CT结果显示手术组心肌坏死部位的99m锝-葡糖二酸的放射性摄取率（心肝比值 1.90±0.33）明显高于正常组大鼠（P< 0.05），利用小动物SPECT/CT融合技术定位的缺血坏死心肌范围和TTC染色法的测量结果呈线性相关（R2=0.964）。结论 通过99m锝-葡糖二酸可以特异性地标记急性缺血坏死心肌，利用小动物SPECT/CT融合技术可以无创性地分析急性缺血坏死心肌的位置和范围。
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| 关 键 词： | 大鼠 缺血/再灌注损伤 99m锝 葡糖二酸 SPECT/CT |
| 收稿时间： | 2014/4/10 0:00:00 |
| 修稿时间： | 2014/5/20 0:00:00 |
In vivo imaging of 99mTc-Glucarate-labeled acute necrotic myocardium using microSPECT/CT |
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| baoluer,ZhangLin,ZhouXiaoHui,MengQingShu,ZhanZhenZhen and FanHuiMin.In vivo imaging of 99mTc-Glucarate-labeled acute necrotic myocardium using microSPECT/CT[J].Chinese Journal of Comparative Medicine,2014,24(7):14-18. |
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| Authors: | baoluer ZhangLin ZhouXiaoHui MengQingShu ZhanZhenZhen and FanHuiMin |
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| Institution: | Department of Cardiac Surgery, Shanghai East Hospital, TongJi University, Shanghai 200120, China;Department of Cardiac Surgery, Shanghai East Hospital, TongJi University, Shanghai 200120, China;Heart Failure department, Shanghai East Hospital, Tongji University, Shanghai 200120, China;Heart Failure Institute, Shanghai East Hospital, Tongji University, Shanghai 200120, China;Department of Cardiac Surgery, Shanghai East Hospital, TongJi University, Shanghai 200120, China;Heart Failure department, Shanghai East Hospital, Tongji University, Shanghai 200120, China;Heart Failure Institute, Shanghai East Hospital, Tongji University, Shanghai 200120, China;Department of Cardiac Surgery, Shanghai East Hospital, TongJi University, Shanghai 200120, China;Heart Failure department, Shanghai East Hospital, Tongji University, Shanghai 200120, China;Heart Failure Institute, Shanghai East Hospital, Tongji University, Shanghai 200120, China;Department of Cardiac Surgery, Shanghai East Hospital, TongJi University, Shanghai 200120, China;Heart Failure department, Shanghai East Hospital, Tongji University, Shanghai 200120, China;Heart Failure Institute, Shanghai East Hospital, Tongji University, Shanghai 200120, China;Department of Cardiac Surgery, Shanghai East Hospital, TongJi University, Shanghai 200120, China;Heart Failure department, Shanghai East Hospital, Tongji University, Shanghai 200120, China;Heart Failure Institute, Shanghai East Hospital, Tongji University, Shanghai 200120, China |
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| Abstract: | Objective To evaluate the anatomic localization and size of acute necrotic myocardium in the ischemic-reperfused rat hearts using 99mTC-Glucarate and microSPECT/CT. Methods The ischemic-reperfused (IR) rat heart models were established by ligating left anterior descending coronary artery for 60 min. 99mTC-Glucarate was intravenously injected into the rats 24 hours after IR operations. Images were acquired 30 min after administration of 99mTC-Glucarate using microSPECT/CT. Anatomic localization and size of acute necrotic myocardium were analyzed with microSPECT/CT imaging,and these results were compared to those determined by triphenyltetrazolium chloride (TTC) staining. Results The microSPECT/CT images showed hot spot accumulations of 99mTC-Glucarate in IR hearts (the heart-to-liver ratio was 1.90±0.33), not in controls (P < 0.05). The anatomic localization of 99mTC-Glucarate-labeled necrotic myocardium were in correspondence with TTC staining results. The hot spot size was related significantly to necrotic myocardial size determined by TTC staining (R2=0.964). Conclusions The localization and size of acute necrotic myocardium can be assessed by non-invasive microSPECT/CT imaging with 99mTc-Glucarate. |
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| Keywords: | Rats Ischemic-reperfusion injury 99mTc Glucarate microSPECT/CT |
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