莱菔子提取液中萝卜苷的大鼠肠吸收特性考察

目的:考察莱菔子水提液中萝卜苷在大鼠小肠的吸收特性。方法:采用大鼠在体单向肠灌流法考察不同药物质量浓度(0.02,0.05,0.1 g·mL-1)对萝卜苷肠吸收特性的影响,计算吸收速率常数(Ka)和表观吸收系数(Papp)。采用HPLC测定萝卜苷含量,流动相0.1%磷酸溶液-乙腈(87∶13),检测波长225 nm。结果:萝卜苷在pH 5.12的K-R试液中稳定性最好。不同质量浓度的莱菔子水提液中萝卜苷在大鼠小肠的Ka分别为(7.750±1.744)×10-3,(7.204±1.045)×10-3,(7.148±0.837)×10-3min-1,Papp分别为(1.519±0.507)×10-3,(1.504±0.617)×10-3,(1.592±0.345)×10-3cm·min-1,均无显著性变化。结论:萝卜苷为易吸收成分,在大鼠小肠中的吸收机制为被动扩散。

Investigation of Intestinal Absorption Characteristics in Rats of Glucoraphenin in Raphani Semen Extract

Objective: To investigate small intestine absorption characteristics in rats of glucoraphenin in Raphani Semen extract. Method: Taking in situ rat single-pass intestinal perfusion model, effects of different drug mass concentrations (0.02, 0.05, 0.1 g· mL^-1) on intestinal absorption characteristics of glucoraphenin was investigated, and calculated their absorption rate constant (K_a) and apparent absorption coefficient (P_app).HPLC was employed to determine the content of glucoraphenin with mobile phase of 0.1% phosphoric acid solution-acetonitrile (87:13) and detectopm wavelength at 225 nm. Result: Glucoraphenin had the best stability in K-R test solution with pH of 5.12.In Raphani Semen extract with different concentrations, K_a of glucoraphenin in small intestine of rats were (7.750±1.744)×10^-3, (7.204±1.045)×10^-3, (7.148±0.837)×10^-3 min^-1, P_app were (1.519±0.507)×10^-3, (1.504±0.617)×10^-3, (1.592±0.345)×10^-3 cm· min^-1, these data had no significant changes. Conclusion: Glucoraphenin was easy-to-absorped component, its absorption mechanism in small intestine of rats was passive diffusion.