长叶胡颓子不同生长部位体内外抗氧化活性及抗LDL氧化修饰的比较

目的:比较长叶胡颓子地上部位与地下部位体内外抗氧化作用,进行长叶胡颓子地上部位开发利用价值及抗动脉粥样硬化(AS)的可行性评价。方法:测定长叶胡颓子各部位在低、中、高质量浓度(5,10,20 g·L-1)对羟自由基(·OH)和超氧阴离子自由基(O-·2)的清除率;观察各部位在低、中、高质量浓度(5,10,20 g·L-1)对铜离子诱导的健康人静脉血低密度脂蛋白(LDL)氧化的影响,测定LDL氧化延迟时间(lag time)和达最大氧化速率时间(Tmax),硫代巴比妥酸(TBA)比色法测定反应液中丙二醛(MDA)含量;选用KM小鼠颈背部皮下注射5%D-半乳糖造模8周后,灌胃给予低、中、高剂量(2,5,10 g·kg-1)长叶胡颓子各部位提取液,14 d后眼眶取血,分离血清,观察长叶胡颓子各部位对血清总抗氧化能力(TAC)、谷胱甘肽过氧化物酶(GPX)、超氧化物歧化酶(SOD)等抗氧化酶活性、丙二醛(MDA)含量的影响。结果:长叶胡颓子地上部位低、中、高各剂量组对O-·2清除率分别为(58.26±6.14)%,(68.21±3.47)%和(74.67±4.10)%,对·OH清除率分别为(48.24±6.24)%,(78.21±7.24)%和(90.17±6.58)%,二者对自由基的清除率均高于地下部位各剂量组,同时长叶胡颓子地上部位和地下部位均能显著增加LDL的Lag time和Tmax,其结果与其减少铜离子诱导的MDA生成作用相一致;血清TAC,SOD,GPX活性均升高,能有效降低小鼠血清MDA含量,其地上部位活性升高显著。结论:长叶胡颓子各部位具有良好的体内外抗氧化活性,能明显提高血清及LDL抗氧化能力,同时长叶胡颓子地上部位抗氧化活性显著优于地下部位。

Antioxidant Activity of Different Part and Anti-LDL Oxidative Modification of Elaeagnus bockii in vitro and in vivo

Objective: To study antioxidant activity of the different part of Elaeagnus bockii in vitro and in vivo, and evaluate the value of development and utilization of aboveground parts of elaeagnus and anti-atherogenic effects. Method: Evaluation of its antioxidant activity in vitro were based on effect of copper ion-induced low density lipoprotein(LDL)oxidation and· OH and O₂ ^. scavenging at low, middle and high concentration(5, 10, 20 g· L^-1). The model KM mice were induced by injecting subcutaneous 5% D-galactose for 8 weeks. Intragastric administration for 14 days and then the orbital blood was taken to test total antioxidant capacity (TAC), superoxide dismutase(SOD), glutathione peroxidase(GPX) antioxidant enzymes activities and malonyldialdehyde(MDA) content in serum(2, 5, 10 g· kg^-1). Result: The rate of free radical scavenging for O₂ ^. at different concentration of Root and rhizome(UP) part were (58.26±6.14)%, (68.21±3.47)%, (74.67±4.10)%, the radical scavenging for· OH were(48.24±6.24)%, (78.21±7.24)%, (90.17±6.58)%. The free radical scavenging rate of the ground part was higher than that of underground parts. Extracts from different parts significantly increased oxidation of delay time(Lag time) and the maximum rate of oxidation time (T_max). The result was consistent with reduced MDA formation induced by copper ion. Serum TAC, SOD, GPX activity of antioxidant enzymes could be increased obviously, and decreased the content of MDA in serum of mice. Conclusion: Different part of E. bockii has good antioxidant activities in vitro and in vivo, significantly increase LDL and serum antioxidant capacity. Antioxidant activity of elaeagnus on aerial parts is significantly superior to the underground parts, which can be developed from above ground parts of elaeagnus for its great development value.