| HPLC法测定复方烧伤膏中没食子酸的含量 |
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| 引用本文： | 张雅,曹蔚,谢艳华,王剑波,胡大海,胡晓龙,缪珊. HPLC法测定复方烧伤膏中没食子酸的含量[J]. 西北药学杂志, 2012, 27(5): 425-426 |
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| 作者姓名： | 张雅 曹蔚 谢艳华 王剑波 胡大海 胡晓龙 缪珊 |
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| 作者单位： | 第四军医大学药学系药物研究所;第四军医大学西京医院烧伤与皮肤外科 |
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| 摘 要： | 目的建立高效液相色谱法测定复方烧伤膏中没食子酸的含量。方法色谱柱:Ultimate C18柱(250mm×4.6mm,5μm);流动相:甲醇-0.5mL.L-1磷酸溶液(10∶90);检测波长:270nm;流速:1.0mL.min-1。结果没食子酸进样量在0.183～3.656μg之间与峰面积积分值呈良好的线性关系(r=0.999 7);平均加样回收率为97.3%,RSD为2.5%(n=5)。结论该法准确、简便、重复性好,能有效控制质量,可用于对复方烧伤膏的质量控制。
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| 关 键 词： | 高效液相色谱法 没食子酸 复方烧伤膏 含量测定 |
Determination of the content of gallic acid in Compound Burn Cream by HPLC |
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| ZHANG Ya,CAO Wei,XIE Yanhua,WANG Jianbo,HU Dahai,HU Xiaolong,MIAO Shan. Determination of the content of gallic acid in Compound Burn Cream by HPLC[J]. Northwest Pharmaceutical Journal, 2012, 27(5): 425-426 |
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| Authors: | ZHANG Ya CAO Wei XIE Yanhua WANG Jianbo HU Dahai HU Xiaolong MIAO Shan |
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| Affiliation: | 1(1.Institute of Materia Medica,School of Pharmacy,the Forth Military Medical University,Xi′an 710032,China;2.Department of Burns and Cutaneous Surgery,Xijing Hospital,the Forth Military Medical University,Xi′an 710032,China) |
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| Abstract: | Objective To estabilish a method for determining gallic acid in Compound Burn Cream by HPLC.Methods An HPLC method was developed on an Ultimate column(250 mm×4.6 mm,5 μm) with methanol-0.5 mL·L-1phosphoric acid solution(10∶90) as the mobile phase.The detecting wavelength was set at 270 nm and the flow rate was 1.0 mL·min-1.Results The calibration curve of gallic acid was linear in the range of 0.183-3.656 μg(r=0.999 7) and the average recovery rate was 97.3% with RSD of 2.5%(n=5).Conclusion The method was simple,accurate and reproducible for the quality control of Compound Burn Cream. |
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| Keywords: | HPLC gallic acid Compound Burn Cream content determination |
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