羟基喜树碱对肠癌细胞体外作用的研究

目的探讨羟基喜树碱对肠癌细胞的体外作用。方法 :采用改良的MTT法检测了 78例肠癌细胞对 5种化疗药物的体外敏感性。结果 :78例肠癌细胞对化疗药物的敏感性 ,由高到低依次为羟基喜树碱 (HCPT)、5-氟脲嘧啶 (5 -Fu)、丝裂霉素 (MMC)、平阳霉素 (PYM)、长春新碱 (VCR)。HCPT和 5 -Fu的敏感性差异无显著性 (P >0 .0 5 ) ,但均明显高于MMC、PYM及VCR(均P <0 .0 0 1) ,对 5 -Fu不敏感的病例中 ,5 7.8%对HCPT敏感或中度敏感。结论 :HCPT和 5 -Fu皆可作为肠癌的首选化疗药物 ,如果 5 -Fu耐药 ,则可选用HCPT。正确取材是MTT法成功的关键。

EFFECT OF HYDROXY CAMPTOTHECIN ON COLORECTAL CARCINOMA CELLS IN VITRO

Objective:To explore the effect of hydroxy camptothecin (HCPT) on colorectal carcinoma cells in vitro. Method:The sensitivity of colorectal carcinoma cells to chemotherapeutic agents was detected in 78 cases with reformed MTT method.Results:The colorectal carcinoma cells of 78 cases showed different sensitivity arrayed from strong to mild to following five chemotherapeutic agents:HCPT,5-Fu,MMC,PYM and VCR.The sensitivity of those cells to HCPT and 5-Fu showed no difference( P >0.05) and was stronger than that of MMC,PYM and VCR( P <0.01).Among the cases who showed no sensitivity to 5-Fu,57.8% were strong or averagely sensitive to HCPT. Conclusions:HCPT and 5-Fu can be used as the preferred chemotherapeutic agents.HCPT may be used if the cells do not show sensitivity to 5-Fu.The key of MTT method is correct biopsy.