| DETECTION OF HUMAN CYTOMEGALOVIRUS INFECTION BY DNA-DNA HYBRIDIZATION |
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| 引用本文： | 盛祖杭,陆志檬,王耆煌,赵寿元,张岩,俞善昌,李云珠. DETECTION OF HUMAN CYTOMEGALOVIRUS INFECTION BY DNA-DNA HYBRIDIZATION[J]. 上海第二医科大学学报, 1988, 0(1) |
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| 作者姓名： | 盛祖杭 陆志檬 王耆煌 赵寿元 张岩 俞善昌 李云珠 |
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| 作者单位： | Clinical Laboratory of Virology，SSMU，Shanghai，Clinical Laboratory of Virology，SSMU，Shanghai，Clinical Laboratory of Virology，SSMU，Shanghai，Institute of Genetics，Fudan University，Shanghai，Institute of Genetics，Fudan University，Shanghai，Department of Peadiatrics，Ruijin Hospital，SSMU，Shanghai，Department of Peadiatrics，Ruijin Hospital，SSMU，Shanghai |
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| 摘 要： | A rapid detection method has been established to identify human cytomegalovirus (HCMV) in specimens of urine and white blood cells with use of DNA-DNA hybridization technology. Since radioactive labels should be avoided if possible, the DNA-DNA hybridization procedure has also been modified to employ biotin labelled probe. Preliminary experiments for sensitivity and specificity showed that hybridization with the probe HCMV DNA EcoRI J fragment could detect 0.9 pg (~(32)P-probe) or 90 pg (biotin-probe) homologous DNA; 60 cells (~(32)P-probe) or 325 cells (biotin-probe) of HCMV infected fibroblasts, and 0.3125 TCID_(50) HCMV-AD_(169). The labelled probe failed to hybridize to DNA from ather herpesviruses, uninfected human lung fibroblasts and human placental DNA. Some specimens have been detected for HCMV by DNA hybridization with ~(32)P-probe. Of 76 urine specimens and 46 white blood cell specimens from 51 newborn infants with clinically suspected cogenital HCMV infection, 11 (12%) and 9 (19%) were positive for HCMV DNA, respectively. Of 11 white blood cell specimens from normal women of childbearing age and 47 white blood cell specimens from blood donors, 6 (55%) and 30 (63%) were positive for HCMV DNA, respectively.
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DETECTION OF HUMAN CYTOMEGALOVIRUS INFECTION BY DNA-DNA HYBRIDIZATION |
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| Sheng Zuhang Lu Zhimeng Wang Qihuang Clinical Laboratory of Virology,SSMU,Shanghai Zhao Shouyuan Zhang Yan Institute of Genetics,Fudan University,Shanghai Yu Shanchang Li Yunzhu. DETECTION OF HUMAN CYTOMEGALOVIRUS INFECTION BY DNA-DNA HYBRIDIZATION[J]. Journal of Shanghai Second Medical University(Foreign Language Edition), 1988, 0(1) |
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| Authors: | Sheng Zuhang Lu Zhimeng Wang Qihuang Clinical Laboratory of Virology SSMU Shanghai Zhao Shouyuan Zhang Yan Institute of Genetics Fudan University Shanghai Yu Shanchang Li Yunzhu |
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| Affiliation: | Sheng Zuhang Lu Zhimeng Wang Qihuang Clinical Laboratory of Virology,SSMU,Shanghai Zhao Shouyuan Zhang Yan Institute of Genetics,Fudan University,Shanghai Yu Shanchang Li Yunzhu Department of Peadiatrics,Ruijin Hospital,SSMU,Shanghai |
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| Abstract: | A rapid detection method has been established to identify human cytomegalovirus (HCMV) in specimens of urine and white blood cells with use of DNA-DNA hybridization technology. Since radioactive labels should be avoided if possible, the DNA-DNA hybridization procedure has also been modified to employ biotin labelled probe. Preliminary experiments for sensitivity and specificity showed that hybridization with the probe HCMV DNA EcoRI J fragment could detect 0.9 pg (~(32)P-probe) or 90 pg (biotin-probe) homologous DNA; 60 cells (~(32)P-probe) or 325 cells (biotin-probe) of HCMV infected fibroblasts, and 0.3125 TCID_(50) HCMV-AD_(169). The labelled probe failed to hybridize to DNA from ather herpesviruses, uninfected human lung fibroblasts and human placental DNA. Some specimens have been detected for HCMV by DNA hybridization with ~(32)P-probe. Of 76 urine specimens and 46 white blood cell specimens from 51 newborn infants with clinically suspected cogenital HCMV infection, 11 (12%) and 9 (19%) were positive for HCMV DNA, respectively. Of 11 white blood cell specimens from normal women of childbearing age and 47 white blood cell specimens from blood donors, 6 (55%) and 30 (63%) were positive for HCMV DNA, respectively. |
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| Keywords: | cytomegalovirus DNA hybridization ~(32)phosphorus biotin |
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