分离纯化眼镜蛇蛇毒因子的一种经济方法

将较大批量的眼镜蛇粗毒依次通过ＳｅｐｈａｄｅｘＧ７５，ＤＥＡＥ－ｃｅｌｌｕｌｏｓｅＤＥ５２和Ｂｉｏ－ＧｅｌＰ２００柱层析可高效地分离纯化眼镜蛇蛇毒因子（ＣＶＦ），５０ｇ粗毒可制备电泳纯ＣＶＦ９６０ｍｇ，产率大大高于既往文献报道，整个制备过程仅需时数日。通过ＳＤＳ聚内烯酰胺凝胶电泳测得其分子量为２２６０００，其溶血活性从抗补体活性均与文献报道相近。所以，本实为一种经济，高效的实验室大量制备ＣＶＦ的方法。

An economical method for fractionation and purification ofcobra venom factor from the venom of Naja naja atra

An efficient method for fractionationand purification of cobra venom factor (CVF) fromthe venom of Naja naja atra by successive chromato-graphy on Sephadex G75, DEAE-cellulose DE52 andBioGel P200 columns was reported in detail.Homogeneity of the CVF obtained was shown as asingle band by both immuno-electrophoresis andpolyacrylamide disc-electrophoresis. The yield was1.92%, i.e. 960 mg obtained from 50 g of crudevenom, much higher than that ever reported. Thewhole procedure of fractionation was completed with-in 3 days. Its relative molecular weight was estimatedto be 226000 composed of 3 subunits by SDS-polyacrylamide gel electrophoresis. lts hemolytic andanticomplementary activities were found as potent asreported else where. The newly developed method is ex-pected to be economically significant for mass produc-tion of CVF in laboratory for chronic in vivopharmacologic experiments.