Iron-dextran antibody conjugates: General method for simultaneous staining of two components in high-resolution immunoelectron microscopy |
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| Authors: | Dutton A H Tokuyasu K T Singer S J |
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| Institution: | Department of Biology, University of California at San Diego, La Jolla, California 92093. |
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| Abstract: | We report the preparation and properties of an electron-dense antibody conjugate, made by the covalent bonding of an iron—dextran (Imposil) particle to an antibody molecule. Transmission electron microscopic experiments with the Imposil—antibody conjugates demonstrate their suitability as specific immunostains at high resolution, particularly for simultaneous double staining experiments in conjunction with ferritin—antibody conjugates. |
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