Ultrastructural responses by Golgi apparatus of rat submandibular gland to beta-adrenergic, alpha-adrenergic, and cholinergic stimulation |
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Authors: | J Blomfield J A Simson A M Martinez J R Martinez |
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Institution: | 1. Children''s Medical Research Foundation, Royal Alexandra Hospital for Children, Sydney, New South Wales 2050, Australia;2. Department of Anatomy, Medical University of South Carolina, Charleston, South Carolina 29425 USA;3. Department of Child Health, School of Medicine, University of Missouri, Columbia, Missouri 65201, USA |
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Abstract: | Rat submandibular gland tissue pieces were stimulated in vitro for 30 min with a beta-adrenergic agent or a cyclic AMP analog to stimulate protein secretion, or with alpha-adrenergic or cholinergic agents or a Ca2+ ionophore to stimulate fluid secretion. Acinar cells were examined by transmission electron microscopy. In control tissue, acinar cells showed little evidence of secretory activity. The Golgi apparatus was sparse and was associated with a few small, immature secretory granules with fine fibrillar contents. Following secretory granule discharge stimulated by isoproterenol or dibutyryl cyclic AMP, acinar cells were constricted, and had extensive basolateral membrane folding and tightly packed rough endoplasmic reticulum. Golgi complexes were prominent and had multiple small granules with filamentous contents. After stimulation of fluid secretion by alpha-adrenergic agents (epinephrine, phenylephrine), or cholinergic agents (acetylcholine, carbachol, pilocarpine), or a Ca2+ ionophore (A23187), the Golgi apparatus had compact concave cisternae enclosing aggregates of tubulovesicles. Acinar cells were distended, basolateral membranes were expanded, and rough endoplasmic reticulum was dilated and vesiculated. |
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Keywords: | To whom reprint requests should be addressed: J Blomfield Children's Medical Research Foundation P O Box 61 Camperdown New South Wales 2050 Australia |
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