格列苯脲对失血性休克大鼠肺损伤的保护作用及机制

目的探讨格列苯脲对失血性休克大鼠肺损伤的保护作用及其机制。方法将健康雄性Wistar大鼠24只随机分为对照组（C组）、休克组（S组）和格列苯脲组（G组）。左股动脉抽放血法制作大鼠失血性休克模型，90min后回输血液和生理盐水进行复苏，G组加用格列苯脲治疗。检测失血期及复苏期不同时点平均动脉压（MAP），并检测肺泡灌流液肿瘤坏死因子-α（TNF-α）、白细胞介素-6（IL-6）水平，以及肺组织髓过氧化物酶（MPO）水平和湿／干重（w／D）比值。结果格列苯脲可改善失血性休克复苏期MAP，并可降低失血性休克后复苏期大鼠肺泡灌流液TNF-α、IL-6水平以及肺组织匀浆MPO、W／D值。结论格列苯脲可通过抑制细胞炎症因子的生成、减少氧化损伤而对失血性休克肺损伤起保护作用。

Protective effect and mechanism of Glibenclamide on lung injury in rat with hemorrhagic shock

Objective To investigate the protective effect of Glibenclamide on rats with hemorrhagic shock. Methods 24 male Wistar rats were randomly divided into 3 groups： control group （group C）, shock group （group S） and Gliben- clamide group （group G）. The model of hemorrhagic shock in rats was produced by controlled method. After 90 min, the rats were resuscitated by transfusion of shed blood and normal saline, but rats in group G were treated with Glibenclamide. At different time points after reperfusion, the mean arterial pressure were recorded. The levels of tumor necrosis factor- alpha （TNF-α）, interleukin-6 （IL-6）, myeloperoxidase （MPO） and wet/dry weight ratio in lung were detected. Results Glibenclamide could improve MAP and reduce the level of TNF-α, IL- 6, MPO and W/D ratio in lung in rat model of hemorrhagic shock. Conclusion Glibenclamide has a protection effect on lung injury in rats with hemorrhagic shock by suppressing the production of inflammatory factors and reducing oxidative damage.