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Cryptosporidium parvum Cpn60 targets a relict organelle |
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| Authors: | Riordan Christina E Ault Jeffrey G Langreth Susan G Keithly Janet S |
| Affiliation: | (1) Wadsworth Center, New York State Department of Health, Axelrod Institute for Public Health, 120 New Scotland Avenue, Albany, NY 12208, USA;(2) Department of Biomedical Sciences, SUNY Albany School of Public Health, Albany, NY 12208, USA;(3) Department of Microbiology and Immunology, Uniformed Services University of the Health Sciences, Bethesda, MD 20814, USA |
| Abstract: | Chaperonin 60 (Cpn60) is a well-established marker protein for eukaryotic mitochondria and plastids. In order to determine whether the small double-membrane-bounded organelle posterior to the nucleus in the apicomplexan Cryptosporidium parvum is a mitochondrion, the Cpn60 gene of C. parvum sporozoites (CpCpn60) was analyzed and antibodies were generated for localization of the peptide. Sequence and phylogenetic analyses indicated that CpCpn60 is a mitochondrial isotype and that antibodies against it localize to the rough endoplasmic reticulum-enveloped remnant organelle of C. parvum sporozoites. These data show this organelle is of mitochondrial origin.Communicated by M. Brunner |
| Keywords: | Apicomplexan Relict mitochondrion Chaperonin 60 (Cpn60) |
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