雷公藤内酯醇对大鼠脑局灶性缺血再灌注后脑组织肿瘤坏死因子-α含量变化的影响

目的　观察雷公藤内酯醇 (TL)是否通过降低脑组织内肿瘤坏死因子 (TNF α)含量而减少白细胞浸润 ,从而改善局灶性脑缺血再灌注所致的神经功能缺失。方法　大鼠ipTL 0 .2或 0 .4mg·kg- 1·d- 1,连续 4d。d 4给药前行右侧大脑中动脉缺血 1h再灌注 2 4h。行为观察行大鼠神经功能缺损评分 ;放射免疫法检测缺血再灌注侧大脑皮层TNF α含量。病理切片观察缺血再灌注侧脑微血管内附壁中性粒细胞计数。结果　与损伤模型组比较 ,TL处理组大脑皮质TNF α含量明显减少 ,神经功能受损程度明显改善。脑微血管内附壁中性粒细胞计数明显减少。结论　TL有抑制缺血再灌注脑组织内TNF α生成 ,降低其含量的作用 ,从而抑制白细胞浸润 ,改善受损的神经功能

Influence of triptolide on content of tumor necrosis factor alpha in ratswith cerebral injury after focal ischemia reperfusion

AIM To study whether the improving effect of triptolide (TL) on deficit of neural function in rats with cerebral injury after focal ischemia-reperfusion by reducing the content of tumor necrosis factor-α(TNF-α) in cerebral tissue that inhibiting the infiltration of neutrophil in the cerebral tissues. METHODS TL 0.2 or 0.4 mg&;#8226;kg^-1&;#8226;d^-1 was injected ip for 4 d. At d 4 before the last dose of TL injection, the focal cerebral ischemia-reperfusion model was established with thread embolism of right middle cerebral artery before TL injection at d 4. At the end of 24-h reperfusion after 1-h focal cerebral ischemia, neurological deficit score of rats was evaluated; radioimmunoassay was used to measure content of TNF-α in cortex; histological techniques were used to count leukocyte accumulation and adhesion in microvassels of cerebral tissue. RESULTS Compared with the ischemia reperfusion group, the content of TNF-α was significantly reduced, the deficit of neural function was markedly improved, and the accumulation of leukocyte in microvassels was remarkably reduced in two TL-treated groups. CONCLUSION TL may reduce the content of TNF-α in cortex with focal cerebral ischemia reperfusion in rats, and inhibit the infiltration of neutrophil in the cerebral tissues of rats.