BAG3 down-modulation sensitizes HPV18 HeLa cells to PEITC-induced apoptosis and restores p53 |
| |
Authors: | Roberta Cotugno Anna BasileElena Romano Dario GallottaMaria Antonietta Belisario |
| |
Institution: | Department of Pharmacy, University of Salerno, Via Giovanni Paolo II n.132, Fisciano, 84084, Salerno, Italy |
| |
Abstract: | BAG3 is a multi-functional component of tumor cell pro-survival machinery, and its biological functions have been largely associated to proteasome system. Here, we show that BAG3 down-modulation resulted in reduced cell viability and enhanced PEITC-induced apoptosis largely more extensively in HeLa (HPV18+) rather than in C33A (HPV−) cervical carcinoma cell lines. Moreover, we demonstrate that BAG3 suppression led to a decrease of viral E6 oncoprotein and a concomitant recovery of p53 tumor suppressor, the best recognized target of E6 for proteasome degradation. E6 and p53 expression were modulated at protein level, since their respective mRNAs were unaffected. Taken together our findings reveal a novel role for BAG3 as host protein contributing to HPV18 E6-activated pro-survival strategies, and suggest a possible relevance of its expression levels in drug/radiotherapy-resistance of HPV18-bearing cervical carcinomas. |
| |
Keywords: | BAG3 HeLa cells HPV18 E6 p53 PEITC |
本文献已被 ScienceDirect 等数据库收录! |
|