| 重组人肝细胞生长因子β链体外生物学活性的测定 |
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| 引用本文： | 常冰梅,牛勃,程牛亮,张悦红,王惠珍,解军,杨涛. 重组人肝细胞生长因子β链体外生物学活性的测定[J]. 中国药物与临床, 2003, 3(2): 122-124,T002 |
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| 作者姓名： | 常冰梅 牛勃 程牛亮 张悦红 王惠珍 解军 杨涛 |
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| 作者单位： | 山西医科大学生物化学与分子生物学教研室,030001 |
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| 摘 要： | 目的 建立一种稳定灵敏的方法用于检测重组人肝细胞生长因子β链 (rhHGF β)对原代培养成年大鼠肝细胞的增生作用。方法 利用噻唑蓝 (MTT)比色方法在多种实验条件下对rhHGF β进行生物学活性检测。结果 对于体外原代培养成年大鼠肝细胞 ,rhHGF β可刺激肝细胞的增生 ,且有剂量依赖效应。 结论 确定了原代培养肝细胞检测rhHGF β生物活性的最佳条件方案
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| 关 键 词： | 肝细胞生长因子 MTT检测 |
Bioactivity assay of recombinant human hepatocyte growth factor-β in vitro |
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| CHANG Bing-mei,NIU Bo,CHENG Niu-liang,ZHANG Yue-hong,WANG Hui-zhen,XIE Jun,YANG Tao. Bioactivity assay of recombinant human hepatocyte growth factor-β in vitro[J]. Chinese Remedies & Clinics, 2003, 3(2): 122-124,T002 |
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| Authors: | CHANG Bing-mei NIU Bo CHENG Niu-liang ZHANG Yue-hong WANG Hui-zhen XIE Jun YANG Tao |
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| Affiliation: | CHANG Bing-mei,NIU Bo,CHENG Niu-liang,ZHANG Yue-hong,WANG Hui-zhen,XIE Jun,YANG Tao.Department of Biochemistry and Molecular Biology of Shanxi Medical University,Taiyuan 030001,China |
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| Abstract: | Objective To work out a stable and sensitive method (as for the cell number,culture time,etc) to detect the effect of recombinant human hepatocyte growth factor-β (rhHGF-β) on the multiplication of primary culture of hepatocytes in adult rats.Methods Bioactivity of rhHGF was assayed under various conditions using MTT.Results rhHGF-β stimulated multiplication of primary adult rat hepatocytes.Conclusion The author sifted out an optimal scheme for detecting the bioactivity of rhHGF-β in primary culture of liver cells. |
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| Keywords: | Hepatocyte growth factor MTT assay |
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