MicroRNA-29b在动脉粥样硬化血管中的表达

目的应用荧光定量PCR技术分析MicroRNA-29b（miR-29b）在Apo-E基因敲除小鼠的动脉粥样硬化（atheroscle—rosis）模型斑块血管中的表达情况，探讨miR-29b在动脉粥样硬化发生、发展过程中的作用。方法8只5周龄、雄性SPF级载脂蛋白E基因敲除小鼠（C57BL／6J—ApoE-／-，apolipoproteinE—deficientmice）为实验组（FS组）。8只雄性、SPF级、相同周龄的C57BL／6J小鼠为对照组（NS组）。高脂（1％胆固醇＋15％猪油）喂食15、20周龄取材检测。结果FS组和NS组体重（29．10±2．53）g和（33．20±5．18）羽无明显差异（P〉0．05）；FS组较NS组血清总胆固醇（（13．52±1．58）mmol／L和（2．12±0．35）mmol／L]显著增高（P〈0．01）；FS组主动脉出现典型的无动脉粥样硬化斑块；NS组主动脉管壁正常，无动脉粥样硬化斑块；FS组主动脉弓miR-29b的表达较NS组平均吸光度值：（2．10±0．27）和（17．56±1．07）1明显降低（P〈0．01）。结论在高脂喂食的载脂蛋白E基因敲除小鼠动脉粥样硬化模型中．miR-29低表达可能与动脉粥样硬化斑块的形成有关。

MicroRNA-29b expression in atherosclerotic aorta

Objective To determine the role and relationship of MicroRNA-29b （miR-29b） in the development process with the atherosclerotic were used fluorescence quantitative PCR technical in the expression of miR-29b on atherosclerotic aorta in Apolipoprotein E-deficient mice. Methods 8 five-week-old male mice which were knocked out apo-E gene grade of SPF C57BL/6J were grouped as the experiment group （FS group）. 8 male mlce of the same age grade of SPF C57BL/6J were grouped as the control group （NS group）. Two groups of mice were fed up with high fat diet for fifteen weeks （1%cholesterol＋15%lard）. When the mice were twenty weeks, took some samples and did the test. Results There was no significant difference between FS group and NS group in the body weight （29.10±2.53） g, （33.20±5.18）g]（P 〉 0.05）. Serum total cholesterol was significantly higher in the FS group than that in the NS group （13.52±1.58）mmol/L, （2.12±0.35） mmol/L] （P 〈 0.01）. There were typical atherosclerotic plaque in aorta in the FS group, but they were normal and there were no atherosclerotic plaque in the NS group. The expression of miR-29b in aortic arch was significantly lower in the FS group than that in the NS group IOD： （2.10±0.27）, （17.56±1.07）] （P 〈 0.01）. Conclusion The forming of atherosclerotie plaque in atherosclerosis model which Apolipoprotein E-deficient mice were fed with high fat diet concern to the lower expression of miR-29b.