应用蚕豆根尖微核技术和彗星试验监测扬中地表水遗传毒物污染 …

目的与方法：本文应用蚕豆根尖微核技术和彗星试验对扬中市不同水体的遗传毒物污染状况进行了初步调查。结果：结果表明，扬中市的遗传毒物污染集中在汇集了乡镇工业废水和生活污水的排污河道和死水沟塘，各主要河道及通江闸口基本没有污染。对其中的新坝大桥（基本无污染）、联合粮管所塘（中轻度污染）、扬子河，红星河（严重污染）水样的有机提取物进行人外周血淋巴细胞的彗星试验，以检测其引起的ＤＮＡ损伤。结果表明扬子河、红

STUDY ON MONITORING GENOTOXICANT POLLUTION IN SURFACE WATER OF YANGZHONG CITY USING MICRONUCLEUS TEST AND COMET ASSAY

Purpose and Methods: In this study, the micronucleus test and comet assay of Vice faber were used to screen pollution situation of the surface water of Yangzhong city. Results: The results showed that the water of Yangzi river and Hongxin river was heavily polluted (Pollution Index>3) and pollution of other water body was slight. Using the comet assay to detect the DNA damage induced by the organic extracts from four water samples, the results indicated that both the comet assay and the micronucleus test were able to detect the genotoxic potential of different water body and the comet assay seemed to be more sensitive than micronucleus test. Conclusion: Combination of the comet assay and the micronucleus test might therefore be recommended for monitoring the genotoxicant pollution of water body.