| 克氏锥虫的新过氧化物酶基因克隆表达研究（英文） |
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| 引用本文： | 杨亚明,John Kelly. 克氏锥虫的新过氧化物酶基因克隆表达研究（英文）[J]. 中国热带医学, 2007, 7(6): 876-880 |
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| 作者姓名： | 杨亚明 John Kelly |
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| 作者单位： | 1. 云南省寄生虫病防治所,云南,思茅,665000
2. The Pathogen Molecular Biology Unit,London School of Hygiene & Tropical Medicine.Keppel Street,London WC1E 7HT,UK |
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| 摘 要： | 目的为了探索克氏锥虫的谷胱甘肽依赖的过氧化物酶活性。方法使用基因克隆与表达鉴定的方法学对克氏锥虫谷胱甘肽依赖的过氧化物酶基因克隆和表达。结果一个全新的、18kDa的克氏锥虫单拷贝过氧化物酶基因被完全克隆到E.coli的表达载体pHrcHis上,并测序。蛋白序列分析表明与基因库谷胱甘肽依赖的过氧化物酶有高度的同源性。其表达蛋白被纯化分离,测定其酶活性显示了良好的活性,并能被亚油酸-氢过氧化物饱和。结论结果证实克氏锥虫仍然存在过氧化物酶介导的生物代谢。
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| 关 键 词： | 克氏锥虫 基因克隆 表达 过氧化酶基因 |
| 文章编号： | 1009-9727（2007）6-876-05 |
| 修稿时间： | 2007-03-10 |
Cloning and expression of a novel peroxidase from Trypanosome cruzi |
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| YANG Ya-ming,John Kelly. Cloning and expression of a novel peroxidase from Trypanosome cruzi[J]. China Tropical Medicine, 2007, 7(6): 876-880 |
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| Authors: | YANG Ya-ming John Kelly |
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| Abstract: | Objective To investigate the activity of glutathione-dependent peroxidase in Trypanosome cruzi (T.cruzi). Methods The gene of glutathione-dependent peroxidase in Trypanosome cruzi (T.cruzi) was cloned and expressed and the genome of T.cruzi was anlayzed. Results A single copy gene from T.cruzi tential to encode a novel 18 kDa peroxidase was cloned into E.coli expression vector pHrcHis and sequenced. Analysis of protein sequence indicated that it has high similarity with glutathione peroxidase from GENEBANK database. A recombinant form of the expressed protein in E.coli has been purified, and the activity of peroxidase assayed showing good activity in the presence of glutathione reductase, and it could be saturated by linoleic acid hydroperoxide. Conclusion This results confirm that there is enzyme-mediated peroxide metabolism in T.cruzi. |
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| Keywords: | Trypanosome cruzi Cloning of gene Expression Peroxidase |
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