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Mitochondrial DNA of Chlamydomonas reinhardtii: the DNA sequence of a region showing homology with mammalian URF2
Authors:Elke Pratje  Susanne Schnierer  Bernard Dujon
Institution:(1) Fakultät für Biologie, Universität Bielefeld, Postfach 8640, D-4800 Bielefeld, Germany;(2) Centre de Génétique moléculaire du C.N.R.S., Laboratoire associé à l'Université Pierre et Marie Curie, F-91190 Gif-sur-Yvette, France;(3) Present address: Botanisches Institut der Universität Düsseldorf, Universitätsstr. 1, D-4000 Düsseldorf, Germany
Abstract:Summary A 1.27 kb DNA fragment of the 15 kb DNA of Chlamydomonas reinhardtii has been cloned and sequenced. A 906 bp long open reading frame was found showing homology with the URF2 genes of mammals and insects. This homology is functional evidence for Chlamydomonas reinhardtii 15 kb DNA representing indeed mitochondrial DNA. This is the first report of an URF2 gene in mitochondria of a photosynthetic organism. The absence of a TGA codon within the gene suggests that it is used as stop codon like in higher plants and not as tryptophan like in animal and fungal mitochondria.
Keywords:Mitochondrial DNA of an alga  Chlamydomonas reinhardtii  DNA sequence  URF2
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