Investigation of the actions and antagonist activity of some polyamine analogues in vivo |
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Authors: | Karen M Doyle Graham G Shaw |
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Affiliation: | 1.Department of Pharmacology, School of Pharmacy, Trinity College, 18 Shrewsbury Road, Dublin 4, Ireland |
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Abstract: | - The ability of three putative polyamine antagonists to antagonize behavioural changes induced by spermine was assessed.
- Injection of an excitotoxic dose of spermine (100 μg, i.c.v.) in mice results in the development of a characteristic behavioural profile, which has two temporally distinct phases. The early events include clonic convulsions, and the later, more general excitation, includes tremor and culminates in the development of a fatal tonic convulsion.
- Co-administration of arcaine (25 μg, i.c.v.) potentiated the early phase effects after spermine injection, but antagonized the development of spermine-induced tonic convulsions. A larger dose of arcaine (50 μg, i.c.v.) given alone resulted in the development of spermine-like body tremor and convulsions. It therefore appears that arcaine is not a pure polyamine antagonist in vivo, but may be a partial agonist.
- Similarly, 1,10-diaminodecane appeared to act as a partial agonist in vivo, although it was less potent than arcaine.
- In contrast, diethylenetriamine (DET) effectively inhibited the development of the early effects of spermine, but was ineffective against the spermine-induced CNS excitation and tonic convulsions.
- It is concluded that none of the putative polyamine antagonists tested behaved as effective polyamine antagonists in vivo, although each produced some antagonism.
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Keywords: | Spermine polyamine CNS excitation convulsion N-methyl-D-aspartate receptor arcaine 1 10-diaminodecane diethylenetriamine |
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