Characterization and gene expression analysis of the two main Th17 cytokines (IL-17A/F and IL-22) in turbot,Scophthalmus maximus |
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| Affiliation: | 1. Instituto de Investigaciones Marinas (IIM), CSIC, Eduardo Cabello 6, 36208 Vigo, Spain;2. Scottish Fish Immunology Research Centre, Institute of Biological and Environmental Sciences, University of Aberdeen, Aberdeen AB24 2TZ, UK;1. Department of Gastroenterology, Jinan University of Second Clinical Medical Sciences, Shenzhen Municipal People’s Hospital, No. 1017, East Gate Road, Shenzhen City, Guangdong Province 518020, China;2. Department of General Surgery, Shenzhen Children’s Hospital, No. 7019, Yitian Road, Shenzhen City, Guangdong Province 518026, China;1. Department of Pathology, University of Michigan Medical School, Ann Arbor, MI 48109, USA;2. Internal Medicine, University of Michigan Medical School, Ann Arbor, MI 48109, USA;3. Comprehensive Cancer Center, University of Michigan Medical School, Ann Arbor, MI 48109, USA;4. Student Medical Academia Center and Department of Public Health, Graduate School of Medicine, Yamaguchi University, Ube, Yamaguchi 755-8505, Japan;1. School of Marine Science and Engineering, Qingdao Agricultural University, Qingdao, 266109, China;2. Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao, 266109, China |
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| Abstract: | This report describes the cloning, characterization and gene expression pattern of two Th17 cytokines, interleukin (IL)-17A/F and -22, in turbot Scophthalmus maximus. The turbot IL-17A/F cDNA contains a 516 bp open reading frame encoding a deduced IL-17A/F protein of 171 amino acid (aa) residues, containing a predicted signal peptide of 31 aa. Turbot IL-22 had a 564 bp ORF coding for a 187 aa protein with a 33 aa signal peptide. The turbot IL-22 protein contained a typical IL-10 family signature. Both cytokines had highest expression levels in the intestine followed by head kidney and gills. Stimulation with the Gram negative bacterium Aeromonas salmonicida was able to modulate IL-17A/F and IL-22 expression in head kidney, spleen and liver but not the intestine. PMA and PHA were also able to induce the expression of both cytokines, suggesting that, as expected, T-cells are likely the main producers of these molecules in turbot as in mammals. |
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