Molecular identification and expression of Lyn tyrosine kinase isoforms in marsupials |
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| Affiliation: | 1. Central Queensland University, Centre for Environmental Management, Marsupial Immunology Research Laboratory, Bruce Highway, Rockhampton, Queensland 4702, Australia;2. Central Queensland University, School of Medical and Applied Sciences, Bruce Highway, Rockhampton, Queensland 4702, Australia;1. Endovascular Division, State Research Center Burnasyan FMBC of the FMBA of Russia, Moscow, Russia;2. Department of Neurosurgery, State Research Center Burnasyan FMBC of the FMBA of Russia, Moscow, Russia;3. Neuro ICU, State Research Center Burnasyan FMBC of the FMBA of Russia, Moscow, Russia;4. Neurosurgical Department, FSBI CCH with Outpatient Health Center of the Business Presidential Administration for the President of the Russian Federation, Moscow, Russia;5. Department of Neurosurgery, University of South Florida, Tampa, Florida, USA;1. Department of Microbiology, Sri Venkata Sai Medical College (BS, MS, SA, VK), Mahabubnagar, Telangana, India;1. Department of Microbiology (DS, MRC), Department of Dermatology (VR), Vardhman Mahavir Medical College and Safdarjung Hospital, New Delhi, Center of Advance Research in Medical Mycology (SG, MRS, AC), WHO Collaborating Center for Reference and Research of Fungi of Medical Importance, National Culture Collection of Pathogenic Fungi, Microbiology, Postgraduate Institute of Medical Education and Research, Chandigarh, India;1. Department of Developmental, Molecular, and Chemical Biology, Tufts University School of Medicine, Boston, MA 02111, USA;2. Genetics Program, Sackler School of Graduate Biomedical Sciences, Tufts University, Boston, MA 02111, USA;3. Cell, Molecular, and Developmental Biology, Sackler School of Graduate Biomedical Sciences, Tufts University, Boston, MA 02111, USA |
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| Abstract: | Lyn is a tyrosine kinase molecule required for modulation of signalling cascades in cell populations including B lymphocytes of the mammalian immune system. We have characterised the coding domain of the marsupial lyn gene of two macropod marsupials; the Tammar Wallaby (Macropus eugenii) and the Bridled Nailtail Wallaby (Onychogalea fraenata) and show the co-expression of two Lyn isoforms in cells and tissues of these and three other marsupials (Brush-tail Possum, Trichosurus vulpecula; American Grey Short-tailed Opossum, Monodelphis domestica and Red-tailed Phascogale, Phascogale calura). The predicted Lyn proteins (LynA and LynB) were highly conserved across vertebrate species, with amino acid identities of 94% with their human orthologues and conservation of key tyrosine kinase motifs that suggests that marsupial Lyn most likely functions in cell signalling. Comparison of our cDNA data to annotations for Lyn transcripts (available through the Ensembl Genome Browser) for the Tammar Wallaby confirm splice sites for a number of exons in the wallaby transcript that are missing from the current annotation. This is the first report of the expression of kinase signalling molecules that influence immunity in metatherian mammals and provides key information to support ongoing studies of immune regulation in marsupials. |
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| Keywords: | Immune signalling Lyn Marsupial Protein tyrosine kinase Wallaby |
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