首页 | 本学科首页   官方微博 | 高级检索  
     


Impact of the cryopreservation technique and vascular bed on ovarian tissue transplantation in cynomolgus monkeys
Authors:M. M. Dolmans  M. M. Binda  S. Jacobs  J. P. Dehoux  J. L. Squifflet  J. Ambroise  J. Donnez  C. A. Amorim
Affiliation:1. P?le de Recherche en Gynécologie, Institut de Recherche Expérimentale et Clinique (IREC), Université Catholique de Louvain, Avenue Mounier 52, bte. B1.52.02, 1200, Brussels, Belgium
4. Department of Gynecology, Cliniques Universitaires Saint Luc, Université Catholique de Louvain, 1200, Brussels, Belgium
2. De Duve Institute, Virologie, Université Catholique de Louvain, 1200, Brussels, Belgium
3. Animal House Facility, Université Catholique de Louvain, 1200, Brussels, Belgium
5. Centre de Technologies Moléculaires Appliquées (CTMA), Institut de Recherche Expérimentale et Clinique (IREC), Université Catholique de Louvain, 1200, Brussels, Belgium
6. Société de Recherche pour l’Infertilité, 1150, Brussels, Belgium
Abstract:

Purpose

The aim of this study was to determine the best combination in terms of cryopreservation techniques and vascular bed preparation before grafting in order to obtain functional ovarian tissue after transplantation.

Methods

Five cynomolgus monkeys were used. Strips from 10 ovaries were cryopreserved, 5 by vitrification (V), and 5 by slow-freezing (SF). Pieces of fresh ovarian tissue were used for controls. After 1 month, the strips were autografted to two different vascular beds, healed (HB) or freshly decorticated (FDB), constituting four study groups: SF-HB, SF-FDB, V-HB, and V-FDB. These were compared to fresh tissue. After 6 months, the ovaries were removed and several parameters analyzed: follicle quality, stage, density, proliferation, apoptosis, functionality, vascularization, and fibrosis. Mixed effect linear regression models were built to assess the impact of cryopreservation and vascular bed preparation on ovarian tissue viability and functionality. p values were adjusted for multiple testing using the Benjamini-Hochberg method, and q values < 0.20 were considered significant in order to achieve a 20 % false discovery rate.

Results

Compared to fresh tissue, no difference was observed in the percentage of morphologically normal follicles, while a significant increase was noted in the follicle proliferation rate (41 %, q = 0.19), percentage of antral follicles (12 %, q = 0.14), and number of vessels per area (3.3 times, q = 0.07) in the V-FDB group.

Conclusions

Vitrification associated with FDB vascular bed preparation is the best combination to obtain functional autografted ovarian tissue. Further studies are nevertheless required, with confirmed pregnancies and live births before introducing the procedure into clinical practice.

Electronic supplementary material

The online version of this article (doi:10.1007/s10815-015-0542-y) contains supplementary material, which is available to authorized users.
Keywords:Ovarian tissue   Vitrification   Slow-freezing   Vascular bed   Freshly decorticated bed   Healed bed   Autotransplantation of ovarian tissue   Grafting   Cynomolgus monkey
本文献已被 SpringerLink 等数据库收录！
设为首页 | 免责声明 | 关于勤云 | 加入收藏

Copyright©北京勤云科技发展有限公司  京ICP备09084417号