乳腺癌组织中PTEN的表达及其临床意义

目的研究肿瘤抑制基因PTEN在乳腺癌组织中的表达及临床意义。方法采用免疫组化染色检测146例乳腺癌组织和10例乳腺癌癌旁正常组织PTEN蛋白的表达情况。结果10例乳腺癌癌旁正常组织均有PTEN表达，免疫组化染色较强，PTEN表达于乳腺小叶腺泡上皮细胞及导管上皮细胞的胞质和胞核。146例乳腺癌组织的PTEN阳性表达率为57．5％（84／146），PTEN蛋白表达于癌细胞的胞质和胞核，PTEN表达与乳腺癌原发肿瘤的大小、病理分期以及雌激素受体（ER）、孕激素受体（PR）有关，PTEN高表达的乳腺癌患者2年生存情况明显优于低表达者（P〈0．05），且ER、PTEN同时表达的乳腺癌患者2年无病生存率高于其中之一未表达或均未表达者（P〈0．05）。结论乳腺癌组织中存在肿瘤抑制基因PTEN的表达缺失或减弱，可能与乳腺癌的发生、发展及预后有关。

Expression and significance of tumor suppressor gene PTEN in breast carcinoma

Objective To investigate the expression of tumor suppressor gene PTEN in breast carcinomas and its significance. Methods Immunohistochemical method was used to detect the expression of PTEN gene in 146 cases of breast carcinoma and 10 specimens of normal breast tissue closely adjacent to carcinoma. Results It was showed that PTEN gene was expressed in all 10 specimens of breast tissue closely adjacent to carcinoma. Expression of PTEN was localized in cytoplasm and nuclei of epithelial cells of lobular acini and epithelial cells of ducts. The rate of PTEN expression was 57.5% (84/146) in breast carcinoma. Expression of PTEN was related to tumor size, pathological stage, and the expression of ER and PR of breast cancer. The 2-year disease free survival of PTEN high expression breast cancer patients was superior to those with low expression (P<0.05), and the 2-year disease free survival of both ER and PTEN positive breast cancer patients was higher than those with only ER or PTEN positive or both of them negative breast cancer (P<0.05). Conclusion This study suggested that tumor suppressor gene PTEN was abnormally expressed in breast carcinoma. PTEN probably plays an important role in the tumorigenesis and development and prognosis of breast cancer.