连翘抗菌成份的研究

连翘(Forsythia suspensa Vahl),李时珍称为“疮家圣药”.中医经验称,连翘主治一切炎性疾患,如脓包疮、淋巴腺炎、淋巴管炎、丹毒、疮疖及多种急性传染病,特别是球菌性传染病.刘国声氏报告,连翘对伤寒杆菌,霍乱弧菌,大肠杆菌,葡萄球菌,白喉杆菌等有抗生作用(见表1).药用部份为果壳.过去知连翘叶中合有连翘配醣体 Phillyrin,

A STUDY OF THE BACTERIOSTATIC PRINCIPLE ISOLATED FROM FORSYTHIA SUSPENSA VAHL.(LIEN-CHIAO)

The concentrated alcoholic extract of the capsules of Forsythia suspensa Vahl,was subjected to repeated extraction with water.As a result,an amorphous phenolic compound with an empirical formula of C₁₅H₁₈O₇ was isolated,which was soluble in sodium hydroxide solution and turned FeCl₃ test solution to green.The compound gave an emerald green fluorescence in water.It is hygroscopic,and forms a 1:80 supersaturated solution in water.It is soluble in alcohol,but insoluble in ethyl ether,chloroform or petroleum ether.Its optical activity could not be detected. The compound has not yet been mentioned in literature concerning F.suspensa Vahl.,and hence named Forsythin.It inhibited the growth of Staphylococcus aureus in vitro at a dilution of 1:5120,and Shigella dysteriae at a dilution of 1:1280. In the same alcoholic extract from the plant,a crystalline sterol compound was obtained after alkaline hydrolysis.This sterol gives elongated prismatic crystals of m.p.200—202℃ with a molecular formula C₄₉H_74-80O₆ (759—765).Its optical activity could not be detected,Ar acetyl derivative with a melting point of 147—149℃ was prepared.