IL-8基因多态性与中国人群炎症性肠病风险的相关性

目的 观察白细胞介素8(IL-8)基因多态性与炎症性肠病(IBD)的相关性.方法 应用PCR-RFLP法和PCR-SSP法对183例无关联的IBD患者包括142例溃疡性结肠炎(UC)患者和41例克罗恩病(CD)]和160例健康对照者IL-8基因的5个单核苷酸多态位点(SNPs)(-845(T/C),-738(T/A),-353(A/T),-251(T/A)和+678(T/C))进行分析.结果 单个SNP与IBD发生风险没有关联,IL-8基因-353 A/T、-251 T/A和+678 T/C三位点的特殊单体型AAT在UC患者中的频率明显高于健康对照组(31.O%比23.7%,P=0.046),不过这种单体型在CD患者和对照中的分布没有差别.结论 IL-8基因的单体型AAT与UC发病之间明显存在一定程度的关联(OR=1.441,95%CI1.007～2.063).

Abstract：

Objective To investigate the association of interleukin 8 (IL-8 ) gene polymorphisms with the risks of inflammatory bowel disease( IBD). Methods Single nucleotide polymorphisms(SNPs) of IL-8 gene at -845 T/C, -738 T/A, -353 A/T, -251 T/A and +678 T/C were analyzed in 183 IBD patients. They included Crohn's disease( CD, n =41) , ulcerative colitis( UC, n = 142) and healthy controls (n= 160). The methods of polymerase chain reaction-restriction fragment length polymorphism ( PCR-RFLP) and polymerase chain reaction-sequence specific primers ( PCR-SSP) were employed. Results No association was observed between any of these five SNPs in IL-8 gene with the occurrence of IBD. A specific haplotype AAT(-353 A/T, -251 T/A & +678 T/C) was over-represented in UC cases when compared with controls(31. 0% vs 23.7%, P= 0.046). But the distributions of this haplotype did not show significant difference between CD cases and controls. Conclusion Our data support a significant but modest association between the AAT haplotype of IL-8 gene and UC( OR = 1. 441, 95% CI 1. 007 -2. 063).

Association of IL-8 gene polymorphisms with inflammatory bowel disease in Chinese patients

Objective To investigate the association of interleukin 8 (IL-8 ) gene polymorphisms with the risks of inflammatory bowel disease( IBD). Methods Single nucleotide polymorphisms(SNPs) of IL-8 gene at -845 T/C, -738 T/A, -353 A/T, -251 T/A and +678 T/C were analyzed in 183 IBD patients. They included Crohn's disease( CD, n =41) , ulcerative colitis( UC, n = 142) and healthy controls (n= 160). The methods of polymerase chain reaction-restriction fragment length polymorphism ( PCR-RFLP) and polymerase chain reaction-sequence specific primers ( PCR-SSP) were employed. Results No association was observed between any of these five SNPs in IL-8 gene with the occurrence of IBD. A specific haplotype AAT(-353 A/T, -251 T/A & +678 T/C) was over-represented in UC cases when compared with controls(31. 0% vs 23.7%, P= 0.046). But the distributions of this haplotype did not show significant difference between CD cases and controls. Conclusion Our data support a significant but modest association between the AAT haplotype of IL-8 gene and UC( OR = 1. 441, 95% CI 1. 007 -2. 063).