炎症浸润细胞在曝光和非曝光部位皮肤中的组织学表现

目的 观察、比较曝光及非曝光部位皮肤中炎症浸润细胞的类型、数目,探讨其在光老化过程中的作用.方法 应用免疫组化法分别对23例女性健康志愿者前臂伸侧(曝光)和上臂内侧(非曝光部位)皮肤石蜡标本中的细胞表面抗原CD3、CD45RO、CD68进行检测,计数阳性细胞数目.不同部位阳性细胞数比较采用配对t检验,阳性细胞数与年龄的相关性采用Pearson相关分析.结果 曝光部位皮肤组织CD3、CD45RO、CD68阳性细胞数分别为(48.91±13.17)/mm2、(46.83±12.92)/mm2、(85.43±22.35)/mm2,非曝光部位分别为(40.61±11.57)/mm2、(38.00±10.11)/mm2、(73.48±16.21)/mm2,曝光部位均显著高于非曝光部位(P<0.01或<0.05).曝光部位皮肤组织CD3、CD45RO阳性细胞数与年龄呈正相关(r=0.56、0.56,P<0.01),曝光部位CD68及非曝光部位皮肤组织CD3、CD45RO、CD68阳性细胞数与年龄无相关性.结论 T淋巴细胞、巨噬细胞可能在光老化过程中发挥作用.

Abstract：

Objective To investigate the role of infiltrating inflammatory cells in photoaging process by comparing the type and number of these cells in sun-exposed and-unexposed skin.Methods The expression of CD3,CD45RO and CD68 were detected by immunohistochemieal staining in 46 paraffin-embeded skin samples from the extensor forearms(sun-exposed)and upper-inner arms(sun-unexposed) of 23 healthy female volunteers.The number of positive cells in sun-exposed and -unexposed sites was counted and statistically tested by paired samples t test,and Pearson correlation analysis was performed to assess the relationship between the number of positive cells and age of these volunteers.Results The number of cells positive for CD3,CD45RO and CD68 per square millimetre in sun-exposed skin was significantly higher than that in sun-unexposed skin(48.91±13.173 vs.40.61±11.571,46.83±12.915 vs.38.00±10.109,85.43±22.346 vs.73.48±16.208,respectively,P<0.01 or 0.05).The number of cells positive for CD3 and CD45RO increased significantly with age (r=0.557,0.555,respectively,both P<0.01) in the sun-exposed skin but not in sun-unexposed skin,and the number of CD68-positive cells was uncorrelated with age in either sunexposed or -unexposed skin.Conclusion T lymphocytes and macrophages may play a role in the process of photoaging.

Histological change of infiltrating inflammatory cells in sun-exposed and-unexposed skin

Objective To investigate the role of infiltrating inflammatory cells in photoaging process by comparing the type and number of these cells in sun-exposed and-unexposed skin.Methods The expression of CD3,CD45RO and CD68 were detected by immunohistochemieal staining in 46 paraffin-embeded skin samples from the extensor forearms(sun-exposed)and upper-inner arms(sun-unexposed) of 23 healthy female volunteers.The number of positive cells in sun-exposed and -unexposed sites was counted and statistically tested by paired samples t test,and Pearson correlation analysis was performed to assess the relationship between the number of positive cells and age of these volunteers.Results The number of cells positive for CD3,CD45RO and CD68 per square millimetre in sun-exposed skin was significantly higher than that in sun-unexposed skin(48.91±13.173 vs.40.61±11.571,46.83±12.915 vs.38.00±10.109,85.43±22.346 vs.73.48±16.208,respectively,P<0.01 or 0.05).The number of cells positive for CD3 and CD45RO increased significantly with age (r=0.557,0.555,respectively,both P<0.01) in the sun-exposed skin but not in sun-unexposed skin,and the number of CD68-positive cells was uncorrelated with age in either sunexposed or -unexposed skin.Conclusion T lymphocytes and macrophages may play a role in the process of photoaging.