外周血全基因组DNA甲基化水平与高脂血症发病风险的相关性分析

目的 探讨外周血全基因组DNA甲基化水平与高脂血症发病风险的相关性。方法 选取2019年1月-2021年1月医院门诊患者282例为研究对象,其中高脂血症患者162例(研究组),非高脂血症患者120例(对照组); 采集所有患者晨起空腹外周静脉血,采用高效液相色谱-串联质谱(Liquid chromatography-tandem mass spectrometry,LC-MS/MS)测定2组外周血全基因组DNA甲基化水平; 收集所有患者的相关资料,采用单因素分析影响高脂血症发病的可能相关因素; 采用Logistic多元回归分析影响高脂血症发病的相关因素; 采用Spearman相关性方法分析高脂血症患者外周血全基因组DNA甲基化水平与影响高脂血症发病因素之间的关系,并绘制受试者工作特征曲线(Receiver operating characteristic curve,ROC)分析外周血全基因组DNA甲基化水平对高脂血症的诊断价值。结果 研究组外周血全基因组DNA甲基化水平高于对照组(P<0.05)。研究组BMI≥23 kg/m²、高热量饮食占比高于对照组; 血清总胆固醇(Total cholesterol,TC)、甘油三酯(Triglyceride,TG)、低密度脂蛋白(Low density lipoprotein,LDL)水平均高于对照组; 高密度脂蛋白(High density lipoprotein,HDL)、总胆红素(Total bilirubin,TBIL)水平均低于对照组(P<0.05)。外周血全基因组DNA甲基化水平、TC,TG,HDL,TBIL均是影响高脂血症发病的相关因素(P<0.05)。Spearman相关性分析显示,外周血全基因组DNA甲基化水平与TC,TG水平呈正相关(r=0.321,0.334,P<0.05); 外周血全基因组DNA甲基化水平与HDL,TBIL水平呈负相关(r=-0.352,-0.341,P<0.05)。外周血全基因组DNA甲基化水平诊断高脂血症的最佳截断点为9.51%,ROC曲线下面积(AUC)为0.695,灵敏度为87.04%,特异度为81.67%。结论 高脂血症患者外周血全基因组DNA甲基化水平较高,是影响高脂血症发病的相关因素,与高脂血症发病风险有关,对于高脂血症具有良好诊断价值。

The correlation between the level of whole-genome DNA methylation in peripheral blood and the risk of hyperlipidemia

Objective To investigate the relationship between the level of whole-genome DNA methylation in peripheral blood and the risk of hyperlipidemia.Methods A total of 282 hospital outpatients from January 2019 to January 2021 were selected as the research subjects,including 162 patients with hyperlipidemia(study group)and 120 patients with non-hyperlipidemia(control group).The fasting peripheral venous blood of all patients was collected in the morning.High performance liquid chromatography-tandem mass spectrometry(LC-MS/MS)was used to determine the level of whole-genome DNA methylation in the peripheral blood of the two groups.The relevant data of all patients were collected and analyzed the possible factors affecting the onset of hyperlipidemia by univariate analysis and Logistic multiple regression analysis.Spearman correlation was used to analyze the relationship between the level of peripheral blood whole genome DNA methylation in patients with hyperlipidemia and the risk factors that affect the onset of hyperlipidemia.And a receiver operating characteristic curve(ROC)was drawn to analyze the diagnostic value of peripheral blood whole genome DNA methylation level for hyperlipidemia.Results The level of DNA methylation in the whole genome of the study group was higher than that of the control group(P<0.05).The study group BMI≥23 kg/m²,and the proportion of high-calorie diet was higher than that of the control group.The serum TC,TG,and LDL contents were higher than those of the control group,and the HDL and TBIL contents were lower than the control group(P<0.05).Peripheral blood whole genome DNA methylation level,TC,TG,HDL,TBIL are all related factors affecting the onset of hyperlipidemia(P<0.05).Spearman correlation analysis showed that peripheral blood whole genome DNA methylation level was positively correlated with TC and TG(r=0.321,0.334,P<0.05).Peripheral blood genome DNA methylation levels were negatively correlated with HDL and TBIL(r=-0.352,-0.341,P<0.05).The best cut-off points for the diagnosis of hyperlipidemia by the whole genome DNA methylation level of peripheral blood was 9.51%.The area under the ROC curve(AUC)was 0.695.The sensitivity was 87.04%,and the specificity was 81.67%.Conclusion Higher levels of whole genome DNA methylation in peripheral blood of hyperlipidemia patients is a factor affecting the onset of hyperlipidemia,and has a good diagnostic value for hyperlipidemia.