多巴胺抑制Erastin诱导PC12细胞系铁死亡

目的探讨多巴胺在不同诱导剂导致的嗜铬细胞瘤PC12细胞死亡中的作用。方法应用RT-qPCR鉴定PC12的肿瘤特异性基因和神经相关基因的mRNA表达;通过细胞存活率评价生理浓度范围内(0~100μmol/L)不同浓度(0、6. 25、12. 5、25、50和100μmol/L)的多巴胺对PC12存活的影响,以及对药物诱导的铁死亡、凋亡和坏死的影响;流式细胞仪检测并分析PC12细胞铁死亡的发生和多巴胺保护PC12过程中PI、Annexin V和细胞周期的情况。结果 PC12表达肿瘤特异性基因和部分神经类基因。多巴胺在浓度25、50和100μmol/L时对PC12细胞有杀伤效果。生理浓度多巴胺特异性保护Erastin诱导的PC12细胞铁死亡。多巴胺能有效的降低Erastin造成的PI、Annexin V双阳性细胞上升和S期到G2/M期阻滞。结论生理浓度多巴胺对PC12有细胞毒性,并可以保护PC12抑制Erastin诱导的铁死亡。

Dopamine inhibits erastin-induced ferroptosis in PC12 cell line

Objective To test dopamine effect on different inducers caused pheochromocytoma PC12 cell death.Methods RT-qPCR was used to test gene expression of tumor associated and nerve related markers of PC12 cells;CCK8 was used to evaluate cell viability of different physiological concentrations of dopamine(0,6.25,12.5,25,50,100 μmol/L)treating PC12 cells and dopamine effect on drug induced ferroptosis,necrosis and apoptosis;flow cytometry was used to test cell cycle changes and positive cell rate of PI and Annexin V during dopamine inhibit ferroptosis.Results PC12 cells expressed tumor specific genes and some neural associated markers.Dopamine had cytotoxicity against PC12 cells at 25,50,and 100 μmol/L.The physiological concentration of dopamine specifically exhibits anti Erastin induced ferroptotic activity,but has no significant effect on apoptosis and necrosis.Dopamine can effectively reduce the increase of PI and Annexin V double positive cells and S phase to G2/M phase block caused by erastin.Conclusions The physiological concentration of dopamine is cytotoxic to PC12 cells and can protect PC12 inhibit ferroptosis induced by erastin.