机械创伤升高大鼠心肌细胞凋亡蛋白表达

目的观察钙蛋白酶(calpain)对机械创伤大鼠心肌细胞凋亡蛋白caspase-3和caspase-12表达的影响。方法将大鼠分为对照组、创伤组和calpain抑制剂(MDL28170)干预组(i. p.,24 mg/kg);用Western blot检测caspase-3、caspase-12、calpain-1和calpain-2蛋白表达;用试剂盒检测caspase-3和caspase-12的活性;用real-time PCR检测calpain-1和calpain-2 mRNA表达。结果与对照组相比,大鼠创伤后心肌细胞caspase-3和caspase-12蛋白表达和活性均升高(P<0. 01),calpain-2表达上调(P<0. 01);与创伤组相比,给予抑制剂MDL28170干预后,心肌细胞calpain-2表达下降(P<0. 05),caspase-3和caspase-12蛋白表达和活性均明显降低(P<0. 05)。结论机械创伤所引起的心肌细胞calpain-2表达上调可能是导致凋亡相关蛋白caspase-3和caspase-12表达和活性升高的重要原因。

Mechanical trauma increases expression of cardiomyocyte apoptosis proteins in rats

Objective To investigate the effects of calpain on apoptosis protein expression of caspase-3 and caspase-12 in rat cardiomyocytes after mechanical trauma. Methods The rats were divided into control group,trauma group,and calpain inhibitor (MDL28170) treatment group (i.p.,24 mg/kg). The protein expression of caspase-3,caspase-12,calpain-1 and calpain-2 was detected by Western blot. Caspase-3 and caspase-12 activity was measured by activity detection kits. The mRNA expression of calpain-1 and calpain-2 was detected by real-time PCR. Results Compared with the control group,the expression and activity of caspase-3 and caspase-12 were both increased (P<0.01),and the expression of calpain-2 increased (P<0.01). Compared with the trauma group,the expression of calpain-2,the expression and activity of caspase-3 and caspase-12 were significantly decreased (P<0.05) after intervention with inhibitor MDL28170. Conclusions Up-regulation of calpain-2 in cardiomyocytes induced by mechanical trauma may be an important cause of increasing the expression and activity of caspase-3 and caspase-12.