川崎病对儿童肠道菌群的影响

目的探讨儿童川崎病对肠道菌群的影响。方法 PCR扩增川崎病患儿和健康儿童粪便样本中16S rRNA基因的V1～V2可变区,并在高通量测序后进行细菌学分类。通过α多样性、β多样性、线性判别分析和LEfSe差异分析比较两组间差异。结果川崎病组的肠道菌群丰度(Ace指数)和结构(非加权ANOSIM)均发生了明显改变(分别为P<0.001和P<0.01)。显著差异菌变化为:乳酸杆菌属(P<0.01)、韦荣球菌属(P<0.01)和梭菌属(P<0.001)丰度减少;拟杆菌属(P<0.05)、肠球菌属(P<0.001)和副杆菌属(P<0.001)丰度增加。结论儿童川崎病存在肠道菌群紊乱。该结果为后期的川崎病微生态学研究提供了部分依据。

Effect of Kawasaki disease on intestinal microbiota in children

Objective To explore the effect of Kawasaki disease on intestinal microbiota in children. Methods The V1~V2 variable region in 16S rRNA gene was amplified by PCR from patients fecal samples who suffered from Kawasaki disease, and the samples were analyzed for bacterial taxonomic content with high-throughput sequencing. Microbiota in affected and healthy children were compared by alpha diversity, beta diversity, linear discriminant analysis and LDA effect size analysis. Results The richness (Ace index, P <0.001) and structure (ANOSIM based on unweighted UniFrac distances, P <0.01) of the intestinal microbiota in the Kawasaki disease group had changed significantly. In particular, Lactobacillus ( P <0.01), Veillonella ( P <0.01), and Clostridium ( P < 0.001) were significantly decreased in affected children, while Bacteroides ( P <0.05), Enterococcus ( P <0.001), and Parabacteroides ( P <0.001) were significantly increased. Conclusions Dysbiosis of intestinal microbiota occurs in children with Kawasaki disease. This result provides a partial basis for the later Kawasaki disease microecology research.