正畸力作用下大鼠牙周组织中表皮生长因子的表达

目的 检测正畸力作用下大鼠牙周组织中表皮生长因子（epidermal growth factor,EGF)及其受体的表达与分布，探讨EGF在正畸牙移动过程中的作用及机制。方法 建立大鼠正畸牙移动模型，分别在受力24h及168h处死，制备左侧上颌第一磨牙及牙周组织的石蜡标本，采用免疫组化HI－SABC法进行检测。结果 EGF与EGFR在正畸组大鼠牙周组织中的表达明显强于对照组（P＜0.01)，受力168h组EGF及EGFR表达的强度高于受力24h组（P＜0.01)；同时间组中，张力侧EGF及EGFR的表达高于压力侧（P＜0.01)；EGF、EGFR在正畸组中的表达主要是位于近远中向的张、压力侧，而生理状态下主要位于根尖及根分叉区的牙周膜。结论 正畸牙移动过程中EGF、EGFR的表达有所增强，提示EGF可能参与了正畸牙移动，并且更多地促进了正畸牙骨改建中的骨形成过程。

Expression of epidermal growth factor and epidermal growth factor receptor in rat periodontal tissues during orthodontic tooth movement

Objective To detect the expression and distribution of epidermal growth factor(EGF)and epidermal growth factor receptor (EGFR)in periodontal tissues,and analyze the role of EGF in orthodontic tooth movement. Methods According to Kings methods, 40 g mesial force was applied to pull the left maxillary first molar in the rat. Using immunohistochemical method (HI SABC method) to localize and examine the expression of EGF and EGFR in decalcified alveodental connective tissues at 24 hours and 168 hours of tooth movement.Results EGF and EGFR were stained at some of periodontal ligament of furcation and radical regions in control group These expressions of EGF and EGFR increased in periodontal tissues( P < 0 01), with the expressions at 168 hours higher than those at 24 hours ( P < 0 01). And levels of EGF and EGFR at tension side were higher than those at pressure side at the same time ( P < 0 01).Conclusions Epidermal growth factor participated in the tissues remodeling during orthodontic tooth movement and especially played a more important role in orthodontic bone formation.