高效液相色谱法测定华细辛中马兜铃酸A含量

 目的测定华细辛中马兜铃酸A的含量。方法建立高效液相色谱方法,采用Agilent Zorbax SB-C₁₈色谱柱(4.6mm×250mm,5μm),以乙腈-1%冰醋酸水溶液(39:61)为流动相,流速为0.8mL·min^-1,检测波长为317nm,分别测定20个华细辛样品中马兜铃酸A的含量。结果高效液相色谱法的回收率为98.9%,RSD为2.1%,标准曲线回归方程为y=2.40x+0.267, 在5.2～68ng内呈良好线性关系。结论华细辛中马兜铃酸A含量在6.8～45.4μg·g^-1。

Quantitative analysis of aristolochic acid A in Asarum sieboldii by HPLC

OBJECTIVE To determine aristolochic acid A in Asarum sieboldii Miq. METHODS A HPLC method was established with a Agilent Zorbax SB-C₁₈ column (4.6mm×250mm, 5μm), a mobile phase of acetonitrile-0.1% acetic acid solution (39: 61), a flow rate of 0.8 mL·min^-1 and a detection wavelength of 317 nm in order to determine the contents of aristolochic acid A in the 20 samples of Asarum sieboldii Miq..RESULTS The average recovery rate was 98.9%, RSD was 2.1%, and the calibration curve was y=2.40x+0.267 with a linear range of 5.2 ng to 68 ng. CONCLUSION The concentration of aristolochic acid A is between 6.8 and 45.4μg·g^-1.