| 血管内皮生长因子基因修饰内皮祖细胞对内膜损伤血管修复的影响 |
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| 引用本文： | 赵刚,黄岚,宋耀明,朱光绪,宋明堡. 血管内皮生长因子基因修饰内皮祖细胞对内膜损伤血管修复的影响[J]. 第三军医大学学报, 2008, 30(5): 427-430 |
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| 作者姓名： | 赵刚 黄岚 宋耀明 朱光绪 宋明堡 |
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| 作者单位： | 第三军医大学新桥医院心血管内科,全军心血管病研究所,重庆,400037 |
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| 摘 要： | 目的 探讨人血管内皮细胞生长因子165(hVEGF165)基因转染骨髓源性内皮祖细胞(EPCs)及对EPCs增殖的影响,在体研究VEGF基因修饰EPCs对内膜损伤血管再内皮化的影响.方法 体外分离、培养、鉴定EPCs,脂质体介导pcDNA3-hVEGF165质粒转染组、pcDNA3空质粒转染组、空白对照组.ELISA法检测EPCs表达VEGF蛋白,MTT法检测hVEGF165基因修饰对EPCs增殖的影响.基因修饰EPCs移植到内膜损伤血管模型中,观察基因修饰对EPCs修复内膜损伤的效应.结果 FITC-UEA-Ⅰ和DiⅠ-acLDL荧光双染证实分化的EPCs,脂质体介导pcDNA3-hVEGF165质粒转染组EPCs培养基上清中表达VEGF,hVEGF165基因转染促进EPCs迁移、黏附和增殖.基因修饰EPCs促进损伤内膜修复.结论 EPCs可以成功转染hVEGF165基因,并且促进EPCs的迁移、黏附和增殖,增强EPCs对损伤内膜修复的能力.
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| 关 键 词： | 内皮祖细胞 血管内皮细胞生长因子 基因转染 |
| 文章编号： | 1000-5404（2008）05-0427-04 |
| 收稿时间： | 2007-11-30 |
| 修稿时间： | 2008-01-04 |
Re-endothelialization of VEGF-transfected endothelial progenitor cells in rat artery intimal injury model |
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| ZHAO Gang,HUANG Lan,SONG Yao-Ming,ZHU Guang-Xu,SONG Ming-Bao. Re-endothelialization of VEGF-transfected endothelial progenitor cells in rat artery intimal injury model[J]. Acta Academiae Medicinae Militaris Tertiae, 2008, 30(5): 427-430 |
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| Authors: | ZHAO Gang HUANG Lan SONG Yao-Ming ZHU Guang-Xu SONG Ming-Bao |
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| Affiliation: | Department of Cardiology;Xinqiao Hospital;Third Military Medical University;Chongqing 400037;China |
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| Abstract: | Objective To explore the proliferation of endothelial progenitor cells (EPCs) after the transfection of human VEGF165, and investigate the re-endothelialization of the transfected cells in artery intimal injury rats. Methods After isolated from rat bone marrow, EPCs were cultured and then identified by FITC-UEA-I and Dil-acLDL. EPCs cells were transfected with pcDNA3-hVEGF165 or pcDNA3 by DOTAP Liposome. After transfection, the expression of VEGF was detected by using ELISA.The EPCs proliferation was studie... |
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| Keywords: | endothelial progenitor cells vascular endothelial growth factor gene transfer |
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