| 外周血中乙型肝炎病毒截短型囊膜蛋白基因的克隆化与序列分析 |
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| 引用本文： | 董菁,成军,王勤环,王刚,施双双,刘妍,夏小兵,斯崇文.外周血中乙型肝炎病毒截短型囊膜蛋白基因的克隆化与序列分析[J].中华肝脏病杂志,2001,9(3):163-165. |
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| 作者姓名： | 董菁 成军 王勤环 王刚 施双双 刘妍 夏小兵 斯崇文 |
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| 作者单位： | 1. 解放军第三○二医院传染病研究所基因治疗研究中心
2. 北京大学第一医院感染疾病科 |
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| 基金项目： | 全军留学归国人员科研启动基金(98H038) |
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| 摘 要： | 目的 研究乙型肝炎病毒（HBV）在慢性感染者体内存在的特殊状态。方法 采用特异性引物设计，以多聚酶链反应（PCR）自身患者血清中扩增全S基因片段，测序以明确变异的部位，并与既往已发表的HBV adr亚型进行序列比较。结果 经测序发现慢性HBV感染者体内存有变异病毒株编码的截短型表面抗原中蛋白基因，并存有HBsAg和多聚酶区缺陷型病毒基因。结论 在外周血中存在HBV截短型囊膜蛋白编码基因，可能与患者不良预后有关。
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| 关 键 词： | 乙型肝炎病毒 截短型囊膜蛋白 聚合酶链反应 突变 准种 克隆 序列分析 |
| 修稿时间： | 2000年5月10日 |
Cloning and sequence analysis of truncated S gene from circulation of patients with chronic hepatitis B virus infection |
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| DONG Jing,CHENG Jun,WANG Qinhuan,et al..Cloning and sequence analysis of truncated S gene from circulation of patients with chronic hepatitis B virus infection[J].Chinese Journal of Hepatology,2001,9(3):163-165. |
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| Authors: | DONG Jing CHENG Jun WANG Qinhuan |
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| Institution: | Gene Therapy Research Center, Institute of Infectious Diseases, 302nd Hospital of PLA, Beijing 100039, China. |
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| Abstract: | Objective To find different mutated status of HBV DNA in circulation from chronic HBV patients. Methods Specially designed primers and polymerase chain reaction method were applied to amplify the whole S gene of HBV from the serum of 2 patients. After being sequenced, 4 clones were compared with HBV adr subtype (China strain) to identify the mutant sites. Results Sequencing results implied that there was a truncated large/middle S gene in the serum of the patients. Besides that, HBsAg and HBV DNA polymerase defective clones were also detected. Conclusions Truncated middle S gene is found in the circulation of patients with chronical HBV infection, suggestive of a poor prognosis. |
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| Keywords: | Hepatitis B virus Truncated middle S protein Polymerase chain reaction Mutation Quasispecies |
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