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| 静脉移植CXCR-4基因转染骨髓间充质干细胞在脊髓损伤鼠体内的迁移与分化 | |
| 引用本文： | 宋晨阳,徐皓,陈建梅,潘廷明,姚晓东.静脉移植CXCR-4基因转染骨髓间充质干细胞在脊髓损伤鼠体内的迁移与分化[J].中国组织工程研究与临床康复,2011,15(23). |
| 作者姓名： | 宋晨阳 徐皓 陈建梅 潘廷明 姚晓东 |
| 作者单位： | 1. 福建医科大学附属协和医院,福建省福州市,350001 2. 解放军南京军区福州总医院,福建省福州市,350025 3. 福建中医药大学附属第二人民医院,福建省福州市,350003 |
| 基金项目： | 南京军区"十一?五"医药卫生科研重点基金资助项目，课题名称:调控SDF-1/CXCR-4促进神经样BMSCs定向迁移修复脊髓损伤的实验研究 |
| 摘 要： | 背景:研究发现,基质细胞衍生因子1/CXCR-4轴具有介导骨髓间充质干细胞定向迁移的作用.目的:观察静脉移植CXCR-4基因转染间充质干细胞治疗脊髓损伤的可行性.方法:利用脊髓横切法构建C57BL/6小鼠T10脊髓损伤模型,造模后7 d抽签随机分成3组:实验组经尾静脉注射CXCR-4基因转染同种异体骨髓间充质干细胞悬液,对照组与空白对照组分别注射同种异体骨髓间充质干细胞悬液及无细胞培养基.移植后第7,14,21,28天利用激光共聚焦显微镜、免疫组织化学双标染色法检测脊髓损伤部位绿色荧光蛋白阳性细胞迁移存活及分化情况,并采用BBB评分评估小鼠神经运动功能恢复情况.结果与结论:实验组移植后各时相点损伤灶内绿色荧光蛋白阳性细胞集聚明显,数量不断增多,迁移率明显高于对照组与空白对照组,来源于骨髓间充质干细胞的神经细胞占移植细胞的比例亦高于对照组与空白对照组,小鼠神经运动功能恢复明显.说明静脉移植CXCR-4基因转染的骨髓间充质干细胞表现出对脊髓损伤灶更强的定向迁移能力,且能在损伤灶内存活、分化,发挥修复损伤脊髓作用. |
| 关 键 词： | 定向迁移 间充质干细胞 CXCR-4基因 脊髓损伤 转染 移植 |
Immigration and differentiation of bone marrow mesenchymal stem cells transfected by CXCR-4 gene after intravenous transplantation in mice with spinal cord injury |
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| Song Chen-yang,Xu Hao,Chen Jian-mei,Pan Ting-ming,Yao Xiao-dong.Immigration and differentiation of bone marrow mesenchymal stem cells transfected by CXCR-4 gene after intravenous transplantation in mice with spinal cord injury[J].Journal of Clinical Rehabilitative Tissue Engineering Research,2011,15(23). | |
| Authors: | Song Chen-yang Xu Hao Chen Jian-mei Pan Ting-ming Yao Xiao-dong |
| Institution: | Song Chen-yang1,Xu Hao2,Chen Jian-mei2,Pan Ting-ming3,Yao Xiao-dong2 1Union Hospital of Fujian Medical University,Fuzhou 350001,Fujian Province,China,2Fuzhou General Hospital of Nanjing Military Area Command of Chinese PLA,Fuzhou 350025,3Second Affiliated People's Hospital,Fujian University of Traditional Chinese Medicine,Fuzhou 350003 |
| Abstract: | BACKGROUND:Previous studies demonstrated that stromal cell derived factor-1/CXCR-4 axis system plays a role in mediating bone marrow mesenchymal stem cells'(BMSCs) orient immigration.OBJECTIVE:To investigate the feasibility of BMSCs transfected by CXCR-4 gene after intravenous transplantation for treatment of spinal cord injury(SCI) .METHODS:C57BL/6 mice T10 SCI model was constructed by spinal cord transection method,and at 7 days after modeling,which were randomly divided into 3 groups:experimental group,c... |
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